Synthesis, decoration, and cellular effects of magnetic mesoporous silica nanoparticles

被引：27

作者：

Nyalosaso, J. L. ^{[1
]}

Rascol, E. ^{[1
]}

Pisani, C. ^{[1
,2
]}

Dorandeu, C. ^{[1
]}

Dumail, X. ^{[1
]}

Maynadier, M. ^{[3
]}

Gary-Bobo, M. ^{[4
]}

Him, J. Lai Kee ^{[5
]}

Bron, P. ^{[5
]}

Garcia, M. ^{[4
]}

Devoisselle, J. M. ^{[1
]}

Prat, O. ^{[2
]}

Guari, Y. ^{[1
]}

Charnay, C. ^{[1
]}

Chopineau, J. ^{[1
,6
]}

机构：

[1] Univ Montpellier, ICGM, CNRS UMR 5253, ENSCM, Campus Triolet,Pl Eugene Bataillon, F-34095 Montpellier 5, France

[2] CEA Marcoule, Direct Rech Fondamentale, BIAM, BP 17171, F-30207 Bagnols Sur Ceze, France

[3] NanoMedSyn, 15 Ave Charles Flahault, F-34093 Montpellier, France

[4] UM, Fac Pharm Montpellier, IBMM, CNRS UMR 5247,ENSCM, 15 Ave Charles Flahault, F-34093 Montpellier 05, France

[5] UM, Ctr Biochim Struct, CNRS UMR 5048, INSERM U1054, F-34090 Montpellier, France

[6] Univ Nimes, Rue Georges Salan, F-30000 Nimes, France

来源：

RSC ADVANCES | 2016年 / 6卷 / 62期

关键词：

ONE-STEP SYNTHESIS; DRUG-DELIVERY; SURFACE FUNCTIONALIZATION; BIOCOMPATIBILITY; CORE; SIZE; NANOCRYSTALS; NANOSPHERES; STABILITY; RELEASE;

D O I：

10.1039/c6ra09017f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Mesoporous Silica Nanoparticles (MSN) are now considered as multifunctional platforms for pharmaceutical development. The goal of this study was to optimize a synthesis procedure to obtain reproducible monodisperse magnetic core@shell Fe3O4@MSN with different coatings and study their uptake by cells. 100 nm core@shell nanoparticles with a unique 18 nm magnetic core were synthesized and covered with PEG groups or coated with a lipid bilayer in a controlled manner and their cellular fate was investigated. Both PEG and lipidic coated nanoparticles exhibit a low toxicity when incubated with Hep-G2 cells compared to pristine ones. Furthermore, the different real-time impedance cellular profiles that were observed and the particles uptake by the cells investigated by TEM suggest different internalization mechanisms or uptake kinetics depending on MSN coverage. This study is a first essential step to ensuring the preparation of well-defined nanomaterials for medical applications; it is considered as a crucial step to be able to perform detailed research about cellular trafficking and signaling pathways.

引用

页码：S7275 / S7283

页数：9

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