PSII-efficiency, polysaccharide production, and phenotypic plasticity of Scenedesmus obliquus in response to changes in metabolic carbon flux

被引：21
作者：
Liu, Ying [1 ]
Wang, Wei [1 ]
Zhang, Min [2 ]
Xing, Peng [2 ]
Yang, Zhou [1 ]
机构：
[1] Nanjing Normal Univ, Sch Biol Sci, Jiangsu Prov Key Lab Biodivers & Biotechnol, Nanjing 210046, Jiangsu, Peoples R China
[2] Chinese Acad Sci, Nanjing Inst Geog & Limnol, Nanjing 210008, Peoples R China
来源：
BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 2010年 / 38卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Carbon flux; Colony formation; Glyoxylate; Growth; Polysaccharide; Scenedesmus obliquus; CYANOBACTERIUM ANABAENA-CYLINDRICA; GREEN-ALGA SCENEDESMUS; EXOPOLYSACCHARIDE PRODUCTION; NITROGENASE ACTIVITY; NUTRIENT CONDITIONS; GLYOXYLATE; PHOTOSYNTHESIS; CHLOROPHYCEAE; MORPHOLOGY; FLUORESCENCE;
D O I：
10.1016/j.bse.2010.02.003
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To investigate the response of Scenedesmus obliquus to changes in metabolic carbon flux, S. obliquus was cultured in medium with different concentrations of glyoxylate over 9 days. Results showed that growth rates were not affected in the lower concentration glyoxylate (0.25 and 0.5 mM). However, growth rate of S. obliquus was inhibited in the higher concentration glyoxylate (0.85 and 1.25 mM) during the early phase before recovering at higher densities. Changes in growth rates in different glyoxylate concentrations were in line with changes in F-v/F-m and Phi(PSII). Colony formation was observed in S. obliquus in the four glyoxylate treatments. As a consequence, the mean number of cells per particle of S. obliquus in the glyoxylate treatments were significantly higher than those in the control. The total polysaccharide content of S. obliquus cells increased with increased glyoxylate concentrations. The increased glyoxylate-stimulated polysaccharide levels were directly correlated with colony size of S. obliquus. (C) 2010 Elsevier Ltd. All rights reserved.
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收藏
页码：292 / 299
页数：8
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