The Signaling Mechanism of TGF-β1 Induced Bovine Mammary Epithelial Cell Apoptosis

被引：11
作者：
Di, He-Shuang [1 ]
Wang, Li-Gang
Wang, Gen-Lin [1 ]
Zhou, Lei [1 ]
Yang, Yuan-Yuan [1 ]
机构：
[1] Nanjing Agr Univ, Dept Anim Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China
来源：
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES | 2012年 / 25卷 / 03期
关键词：
TGF-beta(1); ALK-5; p-Smad2/3; Ca2+]i; Mammary Epithelial Cell; ENDOPLASMIC-RETICULUM; DIFFERENTIAL ROLES; EXPRESSION; PROTEINS; GROWTH; GLAND; DEATH; INHIBITOR;
D O I：
10.5713/ajas.2011.11251
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
The present study showed that Transforming growth factor beta 1 (TGF-beta(1)) can induce apoptosis of bovine mammary epithelial cells. This apoptosis was also observed with phosphorylation of Smad2/3 within 0.5-2 h. Afterwards the signal transferred into the nucleus. Moreover, intracellular free Ca2+ concentration was significantly elevated as well as Caspase-3 activated and DNA lysised, thereby inducing the programmed cell death. This signaling pathway of TGF-beta(1) was blocked by SB-431542 (10(-2) mu M) via inhibiting ALK-5 kinase activity which thus reversed the anti-proliferation and apoptosis effect of TGF-beta(1) in mammary epithelial cells. These results indicated that TGF-beta(1), induced apoptosis of bovine mammary epithelial cells through the ALK-5-Smad2/3 pathway, which plays an important role in inhibiting survival of mammary epithelial cells. Moreover, intracellular Ca2+ also played a critical role in TGF-beta(1)-induced cell apoptosis.
引用
收藏
页码：304 / 310
页数：7
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