Increased production of succinic acid in Escherichia coli by overexpression of malate dehydrogenase

被引：42

作者：

Liang, Li-ya ^{[1
]}

Liu, Rong-ming ^{[1
]}

Ma, Jiang-feng ^{[1
]}

Chen, Ke-quan ^{[1
]}

Jiang, Min ^{[1
]}

Wei, Ping ^{[1
]}

机构：

[1] Nanjing Univ Technol, State Key Lab Mat Oriented Chem Engn, Coll Biotechnol & Pharmaceut Engn, Nanjing 210009, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2011年 / 33卷 / 12期

基金：

中国国家自然科学基金;

关键词：

Dual-phase fermentation; Escherichia coli NZN111; Malate dehydrogenase; Succinic acid; MALIC ENZYME; EXPRESSION; GENE;

D O I：

10.1007/s10529-011-0707-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Escherichia coli NZN111 is a double mutant with inactivated lactate dehydrogenase and pyruvate formate-lyase. It cannot utilize glucose anaerobically because of its unusually high intracellular NADH/NAD(+) ratio. We have now constructed a recombinant strain, E. coli NZN111/pTrc99a-mdh, which, during anaerobic fermentation, produced 4.3 g succinic acid l(-1) from 13.5 g glucose l(-1). The NADH/NAD(+) ratio decreased from 0.64 to 0.26. Furthermore, dual-phase fermentation (aerobic growth followed by anaerobic phase) resulted in enhanced succinic acid production and reduced byproduct formation. The yield of succinic acid from glucose during the anaerobic phase was 0.72 g g(-1), and the productivity was 1.01 g l(-1) h(-1).

引用

页码：2439 / 2444

页数：6

共 15 条

[1] TIGHTLY REGULATED TAC PROMOTER VECTORS USEFUL FOR THE EXPRESSION OF UNFUSED AND FUSED PROTEINS IN ESCHERICHIA-COLI [J].