Acitretin and aloe-emodin loaded chitin nanogel for the treatment of psoriasis

被引:81
作者
Divya, G. [1 ]
Panonnummal, Rajitha [1 ]
Gupta, Swati [1 ]
Jayakumar, R. [2 ]
Sabitha, M. [1 ]
机构
[1] Amrita Univ, Amrita Vishwa Vidyapeetham, Amrita Inst Med Sci & Res Ctr, Amrita Sch Pharm, Kochi 682041, Kerala, India
[2] Amrita Univ, Amrita Vishwa Vidyapeetham, Amrita Inst Med Sci & Res Ctr, Amrita Ctr Nanosci & Mol Med, Kochi 682041, Kerala, India
关键词
Acitretin; Aloe-emodin; Chitin nanogel; Toxicity; Psoriasis; CELL-MEDIATED INFLAMMATION; DRUG-DELIVERY; EPIDERMAL DIFFERENTIATION; PUSTULAR PSORIASIS; SKIN; NANOPARTICLES; CHITOSAN; CRYSTAL; MODEL; VERA;
D O I
10.1016/j.ejpb.2016.06.019
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The present study focuses on the development of an effective topical nanogel formulation of two antipsoriatic drugs; Acitretin (Act) and Aloe-emodin (AE) using natural polymer chitin. Simple regeneration chemistry was used to prepare Chitin Nanogel Systems (CNGs). The developed control chitin (CNGs) nanogels, acitretin loaded chitin nanogels (ActCNGs) and aloe-emodin loaded chitin nanogels (AECNGs) were characterized by DLS, SEM, FTIR, XRD and TG-DTA. The systems were found to be spherical in shape with a size range of 98 +/- 10, 138 +/- 8 and 238 +/- 6 nm having zeta potential values of +28 +/- 3, +27 +/- 3 and +25 +/- 6 mV for CNGs, ActCNGs and AECNGs respectively. The in vitro haemolysis assay revealed that all the nanogel systems are blood compatible. The systems exhibited higher swelling and release at acidic pH. The ex vivo skin permeation studies using porcine skin confirmed the higher deposition of the systems at epidermal and dermal layers, which was confirmed further by fluorescent imaging. The in vivo anti-psoriatic activity study using Perry's mouse tail model and skin safety studies confirmed the potential benefit of the system for topical delivery of acitretin and aloe-emodin in psoriasis. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:97 / 109
页数:13
相关论文
共 63 条
[1]   Development, evaluation and clinical studies of Acitretin loaded nanostructured lipid carriers for topical treatment of psoriasis [J].
Agrawal, Yogeeta ;
Petkar, Kailash C. ;
Sawant, Krutika K. .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2010, 401 (1-2) :93-102
[2]  
Aranaz I., 2009, CURRENT CHEM BIOL, V3, P203, DOI 10.2174/2212796810903020203
[3]   Bio-responsive chitin-poly(L-lactic acid) composite nanogels for liver cancer [J].
Arunraj, T. R. ;
Rejinold, N. Sanoj ;
Kumar, N. Ashwin ;
Jayakumar, R. .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2014, 113 :394-402
[4]   Strategies to maximize treatment success in moderate to severe psoriasis: establishing treatment goals and tailoring of biologic therapies [J].
Brezinski, Elizabeth A. ;
Armstrong, April W. .
SEMINARS IN CUTANEOUS MEDICINE AND SURGERY, 2014, 33 (02) :91-97
[5]   Determination of Marked Components -aloin and aloe-emodin- in Aloe vera before and after hydrolysis [J].
Chiang, Hsiu-Mei ;
Lin, Ya-Tze ;
Hsiao, Pei-Ling ;
Su, Yu-Han ;
Tsao, Hui-Ting ;
Wen, Kuo-Ching .
JOURNAL OF FOOD AND DRUG ANALYSIS, 2012, 20 (03) :646-652
[6]   Electrospun emodin polyvinylpyrrolidone blended nanofibrous membrane: a novel medicated biomaterial for drug delivery and accelerated wound healing [J].
Dai, Xin-Yi ;
Nie, Wei ;
Wang, Yong-Chun ;
Shen, Yi ;
Li, Yan ;
Gan, Shu-Jie .
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2012, 23 (11) :2709-2716
[7]   Interaction of nanoparticles and cell-penetrating peptides with skin for transdermal drug delivery [J].
Desai, Pinaki ;
Patlolla, Ram R. ;
Singh, Mandip .
MOLECULAR MEMBRANE BIOLOGY, 2010, 27 (07) :247-259
[8]   Novel carboxymethyl chitin nanoparticles for cancer drug delivery applications [J].
Dev, Ashish ;
Mohan, Jithin C. ;
Sreeja, V. ;
Tamura, H. ;
Patzke, G. R. ;
Hussain, F. ;
Weyeneth, S. ;
Nair, S. V. ;
Jayakumar, R. .
CARBOHYDRATE POLYMERS, 2010, 79 (04) :1073-1079
[9]   Novel self-assembling nanogels: Stability and lyophilisation studies [J].
Doloud-Mahammed, S. ;
Couvreur, P. ;
Gref, R. .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2007, 332 (1-2) :185-191
[10]  
Dubois D.S., 2013, SCI WORLD J