New microbial mannan catabolic pathway that involves a novel mannosylglucose phosphorylase

被引：72

作者：

Senoura, Takeshi ^{[2
]}

Ito, Shigeaki ^{[3
]}

Taguchi, Hidenori ^{[2
]}

Higa, Mariko ^{[1
]}

Hamada, Shigeki ^{[3
,4
]}

Matsui, Hirokazu ^{[3
]}

Ozawa, Tadahiro ^{[5
]}

Jin, Shigeki ^{[2
]}

Watanabe, Jun ^{[2
,6
]}

Wasaki, Jun ^{[2
,7
]}

Ito, Susumu ^{[1
,2
]}

机构：

[1] Univ Ryukyus, Dept Biosci & Biotechnol, Fac Agr, Okinawa 9030213, Japan

[2] Hokkaido Univ, CRIS, Kita Ku, Sapporo, Hokkaido 0010021, Japan

[3] Hokkaido Univ, Res Fac Agr, Biochem Lab, Kita Ku, Sapporo, Hokkaido 0608589, Japan

[4] Natl Agr & Food Res Org, Natl Inst Crop Sci, Tsukuba, Ibaraki 3058518, Japan

[5] Kao Corp, Tochigi Res Labs, Haga, Ichikai 3213497, Japan

[6] Natl Agr & Food Res Org, Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan

[7] Hiroshima Univ, Grad Sch Biosphere Sci, Higashihiroshima 7398521, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 408卷 / 04期

关键词：

Mannosylglucose phosphorylase; Cellobiose; 2-epimerase; Mannobiose; Mannanase; Operon; Bacteroides; CELLOBIOSE 2-EPIMERASE GENE; RUMINOCOCCUS-ALBUS; BACTEROIDES-THETAIOTAOMICRON; GALACTOMANNAN HYDROLYSIS; CELLVIBRIO-JAPONICUS; MANNOSE METABOLISM; ENZYME; EXPRESSION; BINDING; CLONING;

D O I：

10.1016/j.bbrc.2011.04.095

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The consecutive genes BF0771-BF0774 in the genome of Bacteroides fragilis NCTC 9343 were found to constitute an operon. The functional analysis of BF0772 showed that the gene encoded a novel enzyme, mannosylglucose phosphorylase that catalyzes the reaction, 4-O-beta-D-mannopyranosyl-D-glucose + Pi -> mannose-1-phosphate + glucose. Here we propose a new mannan catabolic pathway in the anaerobe, which involves 1,4-beta-mannanase (BF0771), a mannobiose and/or sugar transporter (BF0773), mannobiose 2-epimerase (BF0774), and mannosylglucose phosphorylase (BF0772), finally progressing to glycolysis. This pathway is distributed in microbes such as Bacteroides, Parabacteroides, Flavobacterium, and (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：701 / 706

页数：6

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