Prevention of iron-polyphenol complex formation by chelation in black tea

Fortifying black tea with iron has the potential to reduce the incidence of iron deficiency in the developing world. However, maintaining iron bioavailability and visual appeal presents a serious technical challenge due to the formation of iron-polyphenol complexes. In this study we tested the validity of using competing complexing agents to prevent the formation of iron-polyphenol"complexes in iron fortified black tea. Disodium EDTA was the most successful and was optimized to a 1:2 iron:EDTA molar ratio. Iron bioavailability was then confirmed by testing the amount of iron that remained soluble and unbound to polyphenol after sequential pH changes similar to that experienced during digestion. Therefore, technology based on combining iron with competing chelating agents in tea has the potential to reduce iron deficiency caused by inadequate iron intake through traditional diets.