Rapid identification of the BjRCO gene associated with lobed leaves in Brassica juncea via bulked segregant RNA-seq

Leaf morphology varies significantly among Brassica juncea. However, little is known regarding the causal gene that controls lobed leaf shape in B. juncea. In our study, the BjRCO gene, which is associated with leaf morphological variation, was cloned from chromosome A10 in B. juncea via BSR-seq. Blast analysis suggested that the BjRCO encodes a HD-Zip I transcription factor, which may serve as a positive regulator of lobed leaf formation in leaf mustard. Interestingly, the lobed leaf genes reported in the related species of Brassica (B. rapa, B. napus, and B. juncea) were located in A10. Phylogenetic analysis showed that BjRCO was also homologous to the lobed leaf gene BoLl reported on chromosome 9 in B. oleracea. In addition, qRT-PCR results showed that the expression levels of the BjRCO gene were higher expressed in the inner small lobed leaves than in the little sawtooth-like leaves (LSL). These results of this study indicate that the BjRCO gene is related to lobed leaf formation in B. juncea. These findings help to establish a solid foundation for future research investigating the molecular mechanism governing lobed leaf formation in B. juncea.