RAMAN SPECTROSCOPY STUDY OF HEALTHY AND CANCEROUS HUMAN BREAST TISSUE FOR CANCER DETECTION

The present study focuses on identifying the features and parameters of Raman spectroscopy for the diagnosis of cancer in human breast surgical samples. Thirty-five clinically unprocessed, fresh human breast tissue specimens (20 cancerous and 15 normal tissues) were obtained under a bioethical protocol approved by the institution's ethical committee. Confocal spontaneous Raman spectroscopy in reflection mode was performed using an incident excitation laser monochromatic beam of 532 nm. The differences observed among Raman profiles of cancerous tissues are more prominent compared with normal breast tissues. Notable spectral differences were present in both the absolute and relative intensities of the Raman peaks in the spectral profile. In the present study, three ratios of Raman intensities 1587 cm-1/ 1637 cm-1, 1587 cm-1/ 1000 cm-1 and 1587 cm-1/ 1375 cm-1 with statistically reliable differences are reported in the present study. It is found to be sensitive enough to differentiate between normal and cancerous breast tissues.