High-throughput screening for various classes of doping agents using a new dilute-and-shoot' liquid chromatography-tandem mass spectrometry multi-target approach

被引：67

作者：

Guddat, S. ^{[1
,2
]}

Solymos, E. ^{[3
]}

Orlovius, A. ^{[1
,2
,4
]}

Thomas, A. ^{[1
,2
]}

Sigmund, G. ^{[1
,2
]}

Geyer, H. ^{[1
,2
]}

Thevis, M. ^{[1
,2
]}

Schaenzer, W. ^{[1
,2
]}

机构：

[1] German Sport Univ Cologne, Inst Biochem, D-50933 Cologne, Germany

[2] German Sport Univ Cologne, Ctr Prevent Doping Res, D-50933 Cologne, Germany

[3] Eotvos Lorand Univ, Joint Res & Training Lab Separat Tech, Budapest, Hungary

[4] Univ Bonn, Inst Pharmaceut, Bonn, Germany

来源：

DRUG TESTING AND ANALYSIS | 2011年 / 3卷 / 11-12期

关键词：

sports drug testing; LC-MS; MS; dilute-and-shoot; high-throughput screening; plasma volume expanders; DEHP glucuronides; ANDROGEN RECEPTOR MODULATORS; HUMAN URINE; CONTROL PURPOSES; LC-MS/MS; DIURETICS; CONFIRMATION; METABOLITES; AGONISTS; OPIATES; STIMULANTS;

D O I：

10.1002/dta.372

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new multi-target approach based on liquid chromatography electrospray ionization tandem mass spectrometry (LC-(ESI)-MS/MS) is presented to screen for various classes of prohibited substances using direct injection of urine specimens. With a highly sensitive new generation hybrid mass spectrometer classic groups of drugs for example, diuretics, beta2-agonists stimulants and narcotics are detectable at concentration levels far below the required limits.

引用

页码：836 / 850

页数：15

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