High-throughput screening for various classes of doping agents using a new dilute-and-shoot' liquid chromatography-tandem mass spectrometry multi-target approach

被引：67
作者：
Guddat, S. [1 ,2 ]
Solymos, E. [3 ]
Orlovius, A. [1 ,2 ,4 ]
Thomas, A. [1 ,2 ]
Sigmund, G. [1 ,2 ]
Geyer, H. [1 ,2 ]
Thevis, M. [1 ,2 ]
Schaenzer, W. [1 ,2 ]
机构：
[1] German Sport Univ Cologne, Inst Biochem, D-50933 Cologne, Germany
[2] German Sport Univ Cologne, Ctr Prevent Doping Res, D-50933 Cologne, Germany
[3] Eotvos Lorand Univ, Joint Res & Training Lab Separat Tech, Budapest, Hungary
[4] Univ Bonn, Inst Pharmaceut, Bonn, Germany
来源：
DRUG TESTING AND ANALYSIS | 2011年 / 3卷 / 11-12期
关键词：
sports drug testing; LC-MS; MS; dilute-and-shoot; high-throughput screening; plasma volume expanders; DEHP glucuronides; ANDROGEN RECEPTOR MODULATORS; HUMAN URINE; CONTROL PURPOSES; LC-MS/MS; DIURETICS; CONFIRMATION; METABOLITES; AGONISTS; OPIATES; STIMULANTS;
D O I：
10.1002/dta.372
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A new multi-target approach based on liquid chromatography electrospray ionization tandem mass spectrometry (LC-(ESI)-MS/MS) is presented to screen for various classes of prohibited substances using direct injection of urine specimens. With a highly sensitive new generation hybrid mass spectrometer classic groups of drugs for example, diuretics, beta2-agonists stimulants and narcotics are detectable at concentration levels far below the required limits.
引用
收藏
页码：836 / 850
页数：15
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