Converting colour to length based on the coffeering effect for quantitative immunoassays using a ruler as readout

被引：38

作者：

Zhang, Dagan ^{[1
]}

Gao, Bingbing ^{[1
]}

Chen, Yangtian ^{[1
]}

Liu, Hong ^{[1
]}

机构：

[1] Southeast Univ, State Key Lab Bioelect, Sch Biol Sci & Med Engn, Nanjing 210096, Jiangsu, Peoples R China

来源：

LAB ON A CHIP | 2018年 / 18卷 / 02期

基金：

中国国家自然科学基金;

关键词：

PAPER-BASED ELISA; LINKED IMMUNOSORBENT ASSAYS; ANALYTICAL DEVICES; PH-METER; RING; NANOPARTICLES; FABRICATION; PROTEINS; CRYSTALS; SURFACE;

D O I：

10.1039/c7lc01127j

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a method for converting the colorimetric results of an enzyme-linked immunosorbent assay (ELISA) into length based on the coffee-ring effect, so that the quantitative detection of analytes can be carried out simply using a ruler. The influence of the shape and lamination of the paper strip on the test results is studied. As a demonstration, human IgG is quantitatively analyzed. It is found that the width of the colored stains correlates with the concentration of the analyte which can be measured for quantitative analysis. The method is promising for quantitative point-of-care detection of biomarkers under resource-limited settings.

引用

页码：271 / 275

页数：5

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