TRIM6 interacts with Myc and maintains the pluripotency of mouse embryonic stem cells

被引:43
作者
Sato, Tomonobu [1 ,2 ]
Okumura, Fumihiko [1 ]
Ariga, Tadashi [2 ]
Hatakeyama, Shigetsugu [1 ]
机构
[1] Hokkaido Univ, Dept Biochem, Grad Sch Med, Kita Ku, Sapporo, Hokkaido 0608638, Japan
[2] Hokkaido Univ, Dept Pediat, Grad Sch Med, Kita Ku, Sapporo, Hokkaido 0608638, Japan
关键词
ES cell; NANOG; Myc; TRIM6; Ubiquitin; UBIQUITIN-PROTEIN LIGASE; SELF-RENEWAL; C-MYC; RING FINGER; U-BOX; ES CELLS; FAMILY PROTEINS; DIFFERENTIATION; EXPRESSION; DEGRADATION;
D O I
10.1242/jcs.095273
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The proto-oncogene product Myc is a master regulator of cell proliferation through its specific binding to the E-box motif in genomic DNA. It has been reported that Myc has an important role in the proliferation and maintenance of the pluripotency of embryonic stem (ES) cells and that the transcriptional activity of Myc is regulated by several post-translational modifications, including ubiquitination. In this study, we showed that tripartite motif containing 6 (TRIM6), one of the TRIM family ubiquitin ligases, was selectively expressed in ES cells and interacted with Myc followed by attenuation of the transcriptional activity of Myc. Knockdown of TRIM6 in ES cells enhanced the transcriptional activity of Myc and repressed expression of NANOG, resulting in the promotion of ES cell differentiation. These findings indicate that TRIM6 regulates the transcriptional activity of Myc during the maintenance of ES cell pluripotency, suggesting that TRIM6 functions as a novel regulator for Myc-mediated transcription in ES cells.
引用
收藏
页码:1544 / 1555
页数:12
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