Comparative &ITin vitro&IT propagation of stress tolerant grape (&ITVitis&IT spp.) rootstocks and assessment of clonal fidelity of plantlets

被引：6

作者：

Motha, Kalpana ^{[1
]}

Singh, S. K. ^{[1
]}

Singh, Rakesh ^{[1
,2
]}

Ram, Chet ^{[1
,2
]}

Srivastav, Manish ^{[1
]}

Verma, M. K. ^{[1
]}

Alizadeh, M. ^{[1
,3
]}

Bhardwaj, Ch. ^{[1
,3
]}

Dev, Rahul ^{[1
,4
]}

机构：

[1] ICAR Indian Agr Res Inst, Div Fruits & Hort Technol, New Delhi 110012, India

[2] ICAR Natl Bur Plant Genet Resources, Pusa Campus, New Delhi 110012, India

[3] ICAR IARI, Div Genet, New Delhi, India

[4] ICAR CAZRI RS, Kachh, Gujarat, India

来源：

INDIAN JOURNAL OF HORTICULTURE | 2017年 / 74卷 / 03期

关键词：

Clonal fidelity; comparative multiplication; grape rootstocks; in vitro propagation; MULTIPLICATION; GENOTYPES; RAPD; CV;

D O I：

10.5958/0974-0112.2017.00065.2

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Micropropagation in nine Vitis rootstocks using nodal segments was studied. Optimum in vitro culture establishment was highest on Murashige and Skoog (1962) medium (MS) with 3.0 mgl(-1) BAP + 0.25 mgl(-1) NAA. Rootstock 110 R gave the earliest bud sprouting (4.03 days), whereas, 1616 C and 110 R gave the highest culture establishment (71.11 & 69.67%). The shoot proliferation was most efficient on MS medium + 4.0 mgl(-1) IBA + 0.5 mgl(-1) BAP. Dogridge showed the maximum multiplication rate/ sub-culture (10.07), while 1613 had the minimum (5.07). Half-strength MS medium supplemented with 4.0 mgl(-1) IBA gave good rooting parameters, while half-strength MS medium with 1.5 mgl(-1) IBA+ 1.5 mgl(-1) NAA induced more number of roots. Dogridge and Salt Creek had the higher rooting (77.58 & 74.24%) compared to other genotypes. High ex vitro plantlet survival (82.75%) was noted in 1103 P in glass jars, while 1616 C plantlets took the shortest time (44.40 days) for transfer to glasshouse, Application of two marker (RAPD & ISSR) systems further confirmed the genetic stability of micropropagated plantlets. Based on the overall performance of rootstocks for in vitro multiplication they could be ranged as Dogridge > Salt Creek > V. parviflora > St. George >1616C > 1103P > 140Ru > 110R > 1613C.

引用

页码：317 / 325

页数：9