Glucose biochip:: dual analyte response in connection to two pre-column enzymatic reactions

被引：21

作者：

Wang, J ^{[1
]}

Chatrathi, MP ^{[1
]}

Ibañez, A ^{[1
]}

机构：

[1] New Mexico State Univ, Dept Chem & Biochem, Las Cruces, NM 88003 USA

来源：

ANALYST | 2001年 / 126卷 / 08期

关键词：

D O I：

10.1039/b103193g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This article describes a novel 'Lab-on-a-Chip' protocol generating two electrophoretic peaks for a single analyte, based on the coupling of two different pre-column enzymatic reactions of the same substrate followed by electrophoretic separation of the reaction products. Such operation is illustrated for the measurement of glucose in connection to the corresponding glucose oxidase (GOx) and glucose dehydrogenase (GDH) reactions. The pre-column enzymatic reactions generate hydrogen peroxide and NADH species, that are separated (based on their different charges) and detected at the end-column amperometric detector. The peak current ratio can be used for confirming the peak identity, estimating the peak purity, addressing co-migrating interferences, and deviations from linearity. A driving voltage of 2000 V results in peroxide and NADH migration times of 93 and 260 s, respectively. Factors influencing the unique dual glucose response are examined and optimized. The concept can be extended to different target analytes based on the coupling of two pre-column reactions with electrophoretic separation of the reaction products.

引用

页码：1203 / 1206

页数：4

共 11 条

[1] INTERNAL STANDARDIZATION TECHNIQUE FOR CAPILLARY ZONE ELECTROPHORESIS [J].

DOSE, EV ;

GUIOCHON, GA .

ANALYTICAL CHEMISTRY, 1991, 63 (11) :1154-1158

[2] Lab-on-a-chip: A revolution in biological and medical sciences. [J].

Figeys, D ;

Pinto, D .

ANALYTICAL CHEMISTRY, 2000, 72 (09) :330A-335A

[3]

Freemantle M, 1999, CHEM ENG NEWS, V77, P27, DOI 10.1021/cen-v077n044.p027

[4] Microfluidic assays of acetylcholinesterase inhibitors [J].

Hadd, AG ;

Jacobson, SC ;

Ramsey, JM .

ANALYTICAL CHEMISTRY, 1999, 71 (22) :5206-5212

[5] Microchip device for performing enzyme assays [J].

Hadd, AG ;

Raymond, DE ;

Halliwell, JW ;

Jacobson, SC ;

Ramsey, JM .

ANALYTICAL CHEMISTRY, 1997, 69 (17) :3407-3412

[6] Measurement of enantiomeric purity by ratio chromatograms with a photometric detector using quartz plates as a multiple retarder [J].

Nakazawa, H ;

Yamada, T ;

Watanabe, T ;

Yamamoto, A ;

Matsunaga, A ;

Nishimura, M .

ANALYTICA CHIMICA ACTA, 1999, 396 (2-3) :125-130

[7] LIQUID-CHROMATOGRAPHY ELECTROCHEMISTRY - THIN-LAYER MULTIPLE ELECTRODE DETECTION [J].

ROSTON, DA ;

SHOUP, RE ;

KISSINGER, PT .

ANALYTICAL CHEMISTRY, 1982, 54 (13) :1417-&

[8] Microseparation chips for performing multienzymatic dehydrogenase/oxidase assays: Simultaneous electrochemical measurement of ethanol and glucose [J].

Wang, J ;

Chatrathi, MP ;

Tian, BM .

ANALYTICAL CHEMISTRY, 2001, 73 (06) :1296-1300

[9] Micromachined electrophoresis chips with thick-film electrochemical detectors [J].

Wang, J ;

Tian, BM ;

Sahlin, E .

ANALYTICAL CHEMISTRY, 1999, 71 (23) :5436-5440

[10] Microfabricated electrophoresis chips for simultaneous bioassays of glucose, uric acid, ascorbic acid, and acetaminophen [J].

Wang, J ;

Chatrathi, MP ;

Tian, BM ;

Polsky, R .

ANALYTICAL CHEMISTRY, 2000, 72 (11) :2514-2518

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