Partial purification and characterization of chitinase produced by Bacillus licheniformis B307

被引:45
作者
Akeed, Yasser [1 ]
Atrash, Faiza [1 ]
Naffaa, Walid [2 ]
机构
[1] Univ Damascus, Fac Sci, Plant Biol Dept, Damascus, Syria
[2] Univ Damascus, Fac Agr, Plant Protect Dept, Damascus 30621, Syria
关键词
Biochemistry; Biotechnology; Microbiology; Molecular biology; Plant biology; Chitinase; Bacillus licheniformis; Optimization; Submerged culture; Purification; BIOCONTROL; IDENTIFICATION; OPTIMIZATION; SUBTILIS; CLONING; DISEASE; WASTE;
D O I
10.1016/j.heliyon.2020.e03858
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The optimal conditions required for chitinase production from Bacillus licheniformis B307 strain, obtained from Syrian soil, were studied. Optimization experiments were carried out under submerged fermentation conditions, and colloidal chitin was the source of carbon. Luria broth medium supplied with 0.5% colloidal chitin was the optimum medium for chitinase production. The maximum chitinase yield was obtained at 30 degrees C, pH6, incubation time 14 days, and 150 rpm. The optimum chitinase activity was achieved at 60 degrees C and pH6. The chitinase activity with unmodified medium was 1.9 U/mL which then enhanced about eight folds to reach 14.2 U/mL under optimized submerged fermentation conditions. An extracellular chitinase of Bacillus licheniformis B307 was partially purified using ammonium sulfate precipitation followed by concentration with various sizes of concentrator tubes. The chitinase was partially purified 8.24 fold and specific enzyme activity increased 2.08 fold (2 U/mg). Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of partial purified chitinase exhibited a molecular weight (M-r) near to 36 and 42kDa. These results make it possible to invest in this strain to produce chitinase to be used as antifungal, food additives and other applications.
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页数:7
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