Effects of cadmium on bone microstructure and serum tartrate-resistant acid phosphatase 5b in male rats

被引：15

作者：

Chen, Xiao ^{[1
]}

Zhu, Guoying ^{[1
]}

Shao, Chunlin ^{[1
]}

Jin, Taiyi ^{[2
]}

Tan, Mingguang ^{[3
]}

Gu, Shuzhu ^{[1
]}

Zhang, Yanyan ^{[1
]}

Xiao, Hanfang ^{[1
]}

机构：

[1] Fudan Univ, Inst Radiat Med, Shanghai 200032, Peoples R China

[2] Fudan Univ, Sch Publ Hlth, Shanghai 200032, Peoples R China

[3] Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 200032, Peoples R China

来源：

EXPERIMENTAL BIOLOGY AND MEDICINE | 2011年 / 236卷 / 11期

关键词：

cadmium; bone microstructure; tartrate-resistant acid phosphatase 5b; bone mineral density; microcomputed tomography; ENVIRONMENTAL EXPOSURE; MECHANICAL-PROPERTIES; MICROARCHITECTURE; DENSITY; OSTEOPOROSIS; MARKER; DYSFUNCTION; RESORPTION; FRACTURES; STRENGTH;

D O I：

10.1258/ebm.2011.011104

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

This study investigated the effects of cadmium on bone microstructure and serum tartrate-resistant acid phosphatase 5b (Tracp 5b) in male rats. Sprague-Dawley male rats were divided into three groups that were given CdCl2 by subcutaneous injection at doses of 0, 0.1 and 0.5 mg/kg body weight (bw) for 12 weeks, respectively. Before killing at the 12th week, microcomputed tomography scanning was performed on the proximal tibia, and urine samples were collected from all of the rats. All rats were then killed, and their blood was collected for biomarkers assay. Bone tissues were dissected for mineral density determinations and histology. The concentration of cadmium in the blood, urine and bone of rats treated with cadmium were significantly higher than in the control group. The bone mineral density, bone mineral concentrations and bone microstructure index of rats treated with cadmium at 0.5 mg/kg bw were clearly lower than in the control rats. Histological investigation also revealed damage to the bone microstructure caused by cadmium. Tracp 5b concentrations in rats treated with cadmium were dose dependently higher than the control. The concentration of cadmium in blood, urine and bone was significantly correlated with Tracp 5b and bone microstructure parameters. Cadmium was shown to induce bone microstructure damage, especially to trabecular bone. The elevated concentrations of serum Tracp 5b suggest that bone resorption mediated via osteoclasts is an important mechanism for the toxic effects of cadmium on bone.

引用

页码：1298 / 1305

页数：8

共 42 条

[1] Cadmium-induced effects on bone in a population-based study of women [J].

Akesson, Agneta ;

Bjellerup, Per ;

Lundh, Thomas ;

Lidfeldt, Jonas ;

Nerbrand, Christina ;

Samsioe, Goran ;

Skerfving, Staffan ;

Vahter, Marie .

ENVIRONMENTAL HEALTH PERSPECTIVES, 2006, 114 (06) :830-834

[2] A novel immunoassay for the determination of tartrate-resistant acid phosphatase 5b from rat serum [J].

Alatalo, SL ;

Peng, ZQ ;

Janckila, AJ ;

Kaija, H ;

Vihko, P ;

Väänänen, HK ;

Halleen, JM .

JOURNAL OF BONE AND MINERAL RESEARCH, 2003, 18 (01) :134-139

[3] Low-level cadmium exposure and osteoporosis [J].

Alfvén, T ;

Elinder, CG ;

Carlsson, MD ;

Grubb, A ;

Hellström, L ;

Persson, B ;

Pettersson, C ;

Spång, G ;

Schütz, A ;

Järup, L .

JOURNAL OF BONE AND MINERAL RESEARCH, 2000, 15 (08) :1579-1586

[4] Bone microarchitecture and bone fragility in men:: DXA and histomorphometry in humans and in the orchidectomized rat model [J].

Audran, M ;

Chappard, D ;

Legrand, E ;

Libouban, H ;

Baslé, MF .

CALCIFIED TISSUE INTERNATIONAL, 2001, 69 (04) :214-217

[5] Metal-bone interactions [J].

Berglund, M ;

Åkesson, A ;

Bjellerup, P ;

Vahter, M .

TOXICOLOGY LETTERS, 2000, 112 :219-225

[6] Weakness in the mechanical properties of the femur of growing female rats exposed to cadmium [J].

Brzóska, M ;

Majewska, K ;

Moniuszko-Jakoniuk, J .

ARCHIVES OF TOXICOLOGY, 2005, 79 (05) :277-288

[7] Mechanical properties of femoral diaphysis and femoral neck of female rats chronically exposed to various levels of cadmium [J].

Brzóska, MM ;

Majewska, K ;

Moniuszko-Jakoniuk, J .

CALCIFIED TISSUE INTERNATIONAL, 2005, 76 (04) :287-298

[8] Bone metabolism of male rats chronically exposed to cadmium [J].

Brzóska, MM ;

Moniuszko-Jakoniuk, J .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 2005, 207 (03) :195-211

[9] Low-level lifetime exposure to cadmium decreases skeletal mineralization and enhances bone loss in aged rats [J].

Brzóska, MM ;

Moniuszko-Jakoniuk, J .

BONE, 2004, 35 (05) :1180-1191

[10] Bone microarchitecture as an important determinant of bone strength [J].

Carbonare, LD ;

Giannini, S .

JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION, 2004, 27 (01) :99-105

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