Misuse of Beer-Lambert Law and other calibration curves

被引:13
作者
Delgado, Rosario [1 ]
机构
[1] Univ Autonoma Barcelona, Dept Math, Campus UAB, Cerdanyola Del Valles 08193, Spain
来源
ROYAL SOCIETY OPEN SCIENCE | 2022年 / 9卷 / 02期
关键词
calibration curve; Beer-Lambert Law; spectrometry; linear regression; concentration; absorbance; INVERSE REGRESSION;
D O I
10.1098/rsos.211103
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Calibration curves allow instrument calibration by predicting the concentration of an analyte in a sample from the reading of the instrument. This curve is constructed as the regression straight line that best fits the relationship between some known concentration standards and their respective instrument readings. An example is the Beer-Lambert Law, used to predict the concentration of a new sample from its absorbance obtained by spectrometry. The issue is that usually this methodology is misapplied. In this paper, we want to clarify this point, explaining what the error consists of and how (easily) to fix it, with the intention of ensuring that it does not continue to be reproduced in the experimental scientific work.
引用
收藏
页数:21
相关论文
共 28 条
  • [21] The Bouguer-Beer-Lambert Law: Shining Light on the Obscure
    Mayerhoefer, Thomas G.
    Pahlow, Susanne
    Popp, Juergen
    CHEMPHYSCHEM, 2020, 21 (18) : 2028 - 2028
  • [22] UV/VIS SPECTROMETER DETERMINATION OF CAFFEINE IN GREEN COFFEE BEANS FROM HARARGHE, ETHIOPIA, USING BEER-LAMBERT'S LAW AND INTEGRATED ABSORPTION COEFFICIENT TECHNIQUES
    Demissie, Ephrem G.
    Woyessa, Girma W.
    Abebe, Arayaselassie
    SCIENTIFIC STUDY AND RESEARCH-CHEMISTRY AND CHEMICAL ENGINEERING BIOTECHNOLOGY FOOD INDUSTRY, 2016, 17 (02) : 109 - 123
  • [23] The Lambert-Beer' s Law Characterization of Formal Analysis in Terahertz Spectrum Quantitative Testing
    Su Hai-xia
    Zhang Zhao-hui
    Zhao Xiao-yan
    Li Zhi
    Yan Fang
    Zhang Han
    SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33 (12) : 3180 - 3186
  • [24] Explanation of human skin color by multiple linear regression analysis based on the modified Lambert-Beer law
    Shimada, M
    Masuda, Y
    Yamada, Y
    Itoh, M
    Takahashi, M
    Yatagai, T
    OPTICAL REVIEW, 2000, 7 (04) : 348 - 352
  • [25] Explanation of Human Skin Color by Multiple Linear Regression Analysis Based on the Modified Lambert-Beer Law
    Miho Shimada
    Yuji Masuda
    Yukio Yamada
    Masahide Itoh
    Motoji Takahashi
    Toyohiko Yatagai
    Optical Review, 2000, 7 : 348 - 352
  • [26] PARAFAC under non-negativity constraint is adapted to recover the underlying Beer-Lambert law of the excitation-emission fluorescence matrix measurements acquired from analyte-triggered semiconductor QDs photoluminescence modulation. When and why?
    Mazivila, Sarmento J.
    Soares, Jose X.
    Lapa, Rui A. S.
    Saraiva, M. Lucia M. F. S.
    Fernandes, Jose O.
    Cunha, Sara C.
    Santos, Joao L. M.
    TALANTA, 2025, 281
  • [27] Determination of calibration curves for 131I in thyroid tumour metabolic radiotherapy and other radionuclides used in SPECT imaging
    D. Arginelli
    J. Heikkonen
    A. Miranti
    C. Peroni
    S. Ridone
    L. Vigna
    Journal of Radioanalytical and Nuclear Chemistry, 2009, 282 : 281 - 285
  • [28] Determination of calibration curves for 131I in thyroid tumour metabolic radiotherapy and other radionuclides used in SPECT imaging
    Arginelli, D.
    Heikkonen, J.
    Miranti, A.
    Peroni, C.
    Ridone, S.
    Vigna, L.
    JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, 2009, 282 (01) : 281 - 285