Simultaneous lipidomic analysis of three families of bioactive lipid mediators leukotrienes, resolvins, protectins and related hydroxy-fatty acids by liquid chromatography/electrospray ionisation tandem mass spectrometry

被引:117
作者
Masoodi, Mojgan [1 ]
Mir, Adnan A. [1 ]
Petasis, Nicos A. [2 ,3 ]
Serhan, Charles N. [4 ,5 ]
Nicolaou, Anna [1 ]
机构
[1] Univ Bradford, Sch Pharm, Bradford BD7 1DP, W Yorkshire, England
[2] Univ So Calif, Dept Chem, Los Angeles, CA 90089 USA
[3] Univ So Calif, Locker Hydrocarbon Res Inst, Los Angeles, CA 90089 USA
[4] Harvard Univ, Sch Dent Med, Dept Oral Med Infect & Immun, Boston, MA 02115 USA
[5] Brigham & Womens Hosp, Ctr Expt Therapeut & Reperfus Injury, Dept Anesthesiol Perioperat & Pain Med, Boston, MA 02115 USA
基金
英国惠康基金;
关键词
D O I
10.1002/rcm.3331
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Bioactive lipid mediators derived from polyunsaturated fatty acids (PUFA) exhibit a range of tissue-and cell-specific activities in many physiological and pathological processes. Electrospray ionisation tandem mass spectrometry coupled to liquid chromatography (LC/ESI-MS/MS) is a sensitive, versatile analytical methodology for the qualitative and quantitative analysis of lipid mediators. Here we present an LC/ESI-MS/MS assay for the simultaneous analysis of twenty mono- and poly-hydroxy-fatty acid derivatives of linoleic, arachidonic, eicosapentaenoic and docosahexaenoic acids. The assay was linear over the concentration range 1-100 pg/mu L, whilst the limits of detection and quantitation were 10-20 and 20-50 pg, respectively. The recovery of the extraction methodology varied from 76-122% depending on the metabolite. This system is useful for profiling a range of biochemically related potent mediators including the newly discovered resolvins and protectins, and their precursor hydroxyeicosapentaenoic and hydroxydocosahexaenoic acids, and, consequently, advance our understanding of the role of PUFA in health and disease. Copyright (c)(0 2007 John Wiley & Sons, Ltd.
引用
收藏
页码:75 / 83
页数:9
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