Membrane-rich milk fat diet provides protection against gastrointestinal leakiness in mice treated with lipopolysaccharide

被引:80
作者
Snow, D. R. [1 ]
Ward, R. E. [1 ]
Olsen, A. [2 ]
Jimenez-Flores, R. [3 ]
Hintze, K. J. [1 ]
机构
[1] Utah State Univ, Dept Nutr Dietet & Food Sci, Logan, UT 84322 USA
[2] Utah State Univ, Dept Anim Dairy & Vet Sci, Logan, UT 84322 USA
[3] Calif Polytech State Univ San Luis Obispo, Dept Agr, Dairy Prod Technol Ctr, San Luis Obispo, CA 93407 USA
关键词
inflammation; lipopolysaccharide (LPS); milk fat globule membrane (MFGM); phospholipid; SUPERCRITICAL-FLUID EXTRACTION; ABERRANT CRYPT FOCI; GUT BARRIER DYSFUNCTION; BUTTERMILK CHEESE WHEY; INDUCED COLON TUMORS; POLAR MFGM LIPIDS; GLOBULE-MEMBRANE; CF1; MICE; INTESTINAL-MUCOSA; SIZE DISTRIBUTION;
D O I
10.3168/jds.2010-3886
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Milk fat globule membrane is a protein-lipid complex that may strengthen the gut barrier. The main objective of this study was to assess the ability of a membrane-rich milk fat diet to promote the integrity of the gut barrier and to decrease systemic inflammation in lipopolysaccharide (LPS)-challenged mice. Animals were randomly assigned to one of 2 American Institute of Nutrition (AIN)-76A formulations differing only in fat source: control diet (corn oil) and milk fat diet (anhydrous milk fat with 10% milk fat globule membrane). Each diet contained 12% calories from fat. Mice were fed diets for 5 wk, then injected with vehicle or LPS (10 mg/kg of BW) and gavaged with dextran-fluorescein to assess gut barrier integrity. Serum was assayed for fluorescence 24 h after gavage, and 16 serum cytokines were measured to assess the inflammatory response. Gut permeability was 1.8-fold higher in LPS-challenged mice fed the control diet compared with the milk fat diet. Furthermore, mice fed the milk fat diet and injected with LPS had lower serum levels of IL-6, IL-10, IL-17, monocyte chemotactic protein (MCP)-1, interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha, and IL-3 compared with LPS-injected mice fed the control diet. The results indicate that the membrane-rich milk fat diet decreases the inflammatory response to a systemic LPS challenge compared with corn oil, and the effect coincides with decreased gut permeability.
引用
收藏
页码:2201 / 2212
页数:12
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