Validation of spectrophotometric determination of chlorogenic acid in fermentation broth and fruits

被引：26

作者：

Wang, Xiao ^{[1
]}

Zeng, Zhaoxia ^{[2
,3
]}

Tian, Zhen ^{[1
]}

Sun, Jinliang ^{[1
]}

Li, Youzhi ^{[1
]}

Fan, Xianwei ^{[1
]}

机构：

[1] Guangxi Univ, Coll Life Sci & Technol, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530004, Peoples R China

[2] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China

[3] Chinese Acad Sci, Huanjiang Observat & Res Stn Karst Ecosyst, Huanjiang 547100, Guangxi, Peoples R China

来源：

FOOD CHEMISTRY | 2019年 / 278卷

关键词：

Chlorogenic acid; Ultraviolet-visible spectrum; HPLC; High-throughput; Biological samples; INSOLUBLE-BOUND PHENOLICS; SPECTRAL PROPERTIES; THERMAL-STABILITY; CAFFEIC ACID; ANTIOXIDANT; SYSTEM; RUTIN;

D O I：

10.1016/j.foodchem.2018.11.041

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A fast and accurate ultraviolet-visible (UV-vis) spectrophotometric method was established to determine the presence of chlorogenic acid (CGA) according to potassium ferricyanide-Fe (III) detection system. The reaction temperature and pH level greatly influenced the CGA absorption spectrum. Maximum UV-vis absorption wavelength occurred at 790 nm under the optimum conditions (at 25 degrees C and pH 7.0). The results of UV-vis were further assessed by comparing the results with those of high-performance liquid chromatography (HPLC). The proposed method showed a wide linear sensing range of 10.0-800.0 mu g mL(-1) (R-2 = 0.9996) and a high degree of precision (% R.S.D. < 1.50) and recovery (% R.S.D. > 3.39). Results of CGA and HPLC methods correlated well with each other. The proposed accurate, rapid, sensitive, low-cost, and high-throughput method was successfully used to quantify CGA in fermentation broth and fruits (Mango). Therefore, it may be applied for measuring CGA in biological samples.

引用

页码：170 / 177

页数：8

共 44 条

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