Evaluation of membrane integrity of frozen/thawed deer spermatozoa:: Short communication

被引：0

作者：

Nagy, S

Kovács, A

Zubor, T

Zomborszky, Z

Tóth, J

Horn, P

机构：

[1] Univ W Hungary, Fac Agr Sci, Inst Anim Breeding, H-9200 Mosonmagyarovar, Hungary

[2] Res Inst Anim Breeding & Nutr, Herceghalom, Hungary

[3] Embryo Ltd, Pecs, Hungary

[4] Univ Kaposvar, Fac Anim Sci, Kaposvar, Hungary

[5] Pannon Equestrian Acad, Kaposvar, Hungary

[6] Specialist Secondary Sch Agr, Kaposvar, Hungary

来源：

ACTA VETERINARIA HUNGARICA | 2001年 / 49卷 / 02期

关键词：

deer; spermatozoa; membrane integrity; acrosome;

D O I：

10.1556/004.49.2001.2.12

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A simultaneous live/dead and acrosome staining, originally described for domestic mammals, was successfully applied on red deer (Cervus elaphus) and fallow deer (Dama dama) spermatozoa collected from the cauda epididymidis and vas deferens of shot stags. The staining is simple enough for routine application. Seven classes of spermatozoa were distinguished in the smears of frozen/thawed semen samples. Morphology, including cytoplasmic droplets, was evaluated as well. Percentage of live cells with intact acrosomes and with no other morphological aberrations might be a practical index of semen quality.

引用

页码：223 / 227

页数：5

共 5 条

[1]

Barth A., 1989, ABNORMAL MORPHOLOGY

[2]

Garde JJ, 1997, ARCH ANDROLOGY, V39, P1

[3] VIABILITY AND ACROSOME STAINING OF BULL, BOAR AND RABBIT SPERMATOZOA [J].