Irisin - a myth rather than an exercise-inducible myokine

被引：256

作者：

Albrecht, Elke ^{[1
]}

Norheim, Frode ^{[2
]}

Thiede, Bernd ^{[3
]}

Holen, Torgeir ^{[2
]}

Ohashi, Tomoo ^{[4
]}

Schering, Lisa ^{[1
]}

Lee, Sindre ^{[2
]}

Brenmoehl, Julia ^{[5
]}

Thomas, Selina ^{[6
]}

Drevon, Christian A. ^{[2
]}

Erickson, Harold P. ^{[4
]}

Maak, Steffen ^{[1
]}

机构：

[1] Leibniz Inst Farm Anim Biol, Inst Muscle Biol & Growth, D-18196 Dummerstorf, Germany

[2] Univ Oslo, Inst Basic Med Sci, Dept Nutr, N-0317 Oslo, Norway

[3] Univ Oslo, Biotechnol Ctr Oslo, N-0317 Oslo, Norway

[4] Duke Univ, Dept Cell Biol, Durham, NC 27710 USA

[5] Leibniz Inst Farm Anim Biol, Inst Genome Biol, D-18196 Dummerstorf, Germany

[6] Univ Bern, Vetsuisse Fac, Swiss Inst Equine Med ISME, CH-1580 Avenches, Switzerland

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

关键词：

FNDC5; GENE-EXPRESSION; SKELETAL-MUSCLE; ADIPOSE-TISSUE; CIRCULATING IRISIN; PHYSICAL-ACTIVITY; WHITE FAT; YOUNG; RELEASE; HEALTHY; HUMANS;

D O I：

10.1038/srep08889

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The myokine irisin is supposed to be cleaved from a transmembrane precursor, FNDC5 (fibronectin type III domain containing 5), and to mediate beneficial effects of exercise on human metabolism. However, evidence for irisin circulating in blood is largely based on commercial ELISA kits which are based on polyclonal antibodies (pAbs) not previously tested for cross-reacting serum proteins. We have analyzed four commercial pAbs by Western blotting, which revealed prominent cross-reactivity with non-specific proteins in human and animal sera. Using recombinant glycosylated and non-glycosylated irisin as positive controls, we found no immune-reactive bands of the expected size in any biological samples. A FNDC5 signature was identified at similar to 20 kDa by mass spectrometry in human serum but was not detected by the commercial pAbs tested. Our results call into question all previous data obtained with commercial ELISA kits for irisin, and provide evidence against a physiological role for irisin in humans and other species.

引用

页数：10

共 37 条

[1] Metabolic adaptations in skeletal muscle, adipose tissue, and whole-body oxidative capacity in response to resistance training
Alvehus, Malin
Boman, Niklas
Soderlund, Karin
Svensson, Michael B.
Buren, Jonas
[J]. EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, 2014, 114 (07) : 1463 - 1471
[2] Circulating Irisin in Healthy, Young Individuals: Day-Night Rhythm, Effects of Food Intake and Exercise, and Associations With Gender, Physical Activity, Diet, and Body Composition
Anastasilakis, Athanasios D.
Polyzos, Stergios A.
Saridakis, Zacharias G.
Kynigopoulos, Georgios
Skouvaklidou, Elpida C.
Molyvas, Dimitrios
Vasiloglou, Maria F.
Apostolou, Aggeliki
Karagiozoglou-Lampoudi, Thomai
Siopi, Aikaterina
Mougios, Vassilis
Chatzistavridis, Panagiotis
Panagiotou, Grigorios
Filippaios, Andreas
Delaroudis, Sideris
Mantzoros, Christos S.
[J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2014, 99 (09) : 3247 - 3255
[3] Greek goddess or Greek myth: the effects of exercise on irisin/FNDC5 in humans
Atherton, P. J.
Phillips, B. E.
[J]. JOURNAL OF PHYSIOLOGY-LONDON, 2013, 591 (21): : 5267 - 5268
[4] A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis
Bostroem, Pontus
Wu, Jun
Jedrychowski, Mark P.
Korde, Anisha
Ye, Li
Lo, James C.
Rasbach, Kyle A.
Bostroem, Elisabeth Almer
Choi, Jang Hyun
Long, Jonathan Z.
Kajimura, Shingo
Zingaretti, Maria Cristina
Vind, Birgitte F.
Tu, Hua
Cinti, Saverio
Hojlund, Kurt
Gygi, Steven P.
Spiegelman, Bruce M.
[J]. NATURE, 2012, 481 (7382) : 463 - U72
[5] Irisin Is Elevated in Skeletal Muscle and Serum of Mice Immediately after Acute Exercise
Brenmoehl, Julia
Albrecht, Elke
Komolka, Katrin
Schering, Lisa
Langhammer, Martina
Hoeflich, Andreas
Maak, Steffen
[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 2014, 10 (03): : 338 - 349
[6] A cross-platform toolkit for mass spectrometry and proteomics
Chambers, Matthew C.
Maclean, Brendan
Burke, Robert
Amodei, Dario
Ruderman, Daniel L.
Neumann, Steffen
Gatto, Laurent
Fischer, Bernd
Pratt, Brian
Egertson, Jarrett
Hoff, Katherine
Kessner, Darren
Tasman, Natalie
Shulman, Nicholas
Frewen, Barbara
Baker, Tahmina A.
Brusniak, Mi-Youn
Paulse, Christopher
Creasy, David
Flashner, Lisa
Kani, Kian
Moulding, Chris
Seymour, Sean L.
Nuwaysir, Lydia M.
Lefebvre, Brent
Kuhlmann, Frank
Roark, Joe
Rainer, Paape
Detlev, Suckau
Hemenway, Tina
Huhmer, Andreas
Langridge, James
Connolly, Brian
Chadick, Trey
Holly, Krisztina
Eckels, Josh
Deutsch, Eric W.
Moritz, Robert L.
Katz, Jonathan E.
Agus, David B.
MacCoss, Michael
Tabb, David L.
Mallick, Parag
[J]. NATURE BIOTECHNOLOGY, 2012, 30 (10) : 918 - 920
[7] Implication of Circulating Irisin Levels with Brown Adipose Tissue and Sarcopenia in Humans
Choi, Hae Yoon
Kim, Sungeun
Park, Ji Woo
Lee, Nam Seok
Hwang, Soon Young
Huh, Joo Young
Hong, Ho Cheol
Yoo, Hye Jin
Baik, Sei Hyun
Youn, Byung-Soo
Mantzoros, Christos S.
Choi, Kyung Mook
[J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2014, 99 (08) : 2778 - 2785
[8] Plasma irisin levels progressively increase in response to increasing exercise workloads in young, healthy, active subjects
Daskalopoulou, Stella S.
Cooke, Alexandra B.
Gomez, Yessica-Haydee
Mutter, Andrew F.
Filippaios, Andreas
Mesfum, Ertirea T.
Mantzoros, Christos S.
[J]. EUROPEAN JOURNAL OF ENDOCRINOLOGY, 2014, 171 (03) : 343 - 352
[9] Irisin and FNDC5: effects of 12-week strength training, and relations to muscle phenotype and body mass composition in untrained women
Ellefsen, S.
Vikmoen, O.
Slettalokken, G.
Whist, J. E.
Nygaard, H.
Hollan, I.
Rauk, I.
Vegge, G.
Strand, T. A.
Raastad, T.
Ronnestad, B. R.
[J]. EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, 2014, 114 (09) : 1875 - 1888
[10] COMMENT ON WU AND SPIEGELMAN Irisin ERKs the Fat. Diabetes 2014;63:381-383
Elsen, Manuela
Raschke, Silja
Sommerfeld, Mark
Gassenhuber, Hans
Eckel, Juergen
[J]. DIABETES, 2014, 63 (09) : E16 - E16

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