Inhibition studies of soybean (Glycine max) urease with heavy metals, sodium salts of mineral acids, boric acid, and boronic acids

被引：19
作者：
Kumar, Sandeep [1 ]
Kayastha, Arvind M. [1 ]
机构：
[1] Banaras Hindu Univ, Sch Biotechnol, Fac Sci, Varanasi 221005, Uttar Pradesh, India
来源：
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY | 2010年 / 25卷 / 05期
关键词：
Urease; inhibition; soybean; boric acid; boronic acid; metals; JACK BEAN UREASE; KLEBSIELLA-AEROGENES UREASE; MICROBIAL UREASES; ACTIVE-SITE; IONS; FLUORIDE; SEEDS; INACTIVATION; PURIFICATION; MECHANISM;
D O I：
10.3109/14756360903468155
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Various inhibitors were tested for their inhibitory effects on soybean urease. The K-i values for boric acid, 4-bromophenylboronic acid, butylboronic acid, and phenylboronic acid were 0.20 +/- 0.05 mM, 0.22 +/- 0.04 mM, 1.50 +/- 0.10 mM, and 2.00 +/- 0.11 mM, respectively. The inhibition was competitive type with boric acid and boronic acids. Heavy metal ions including Ag+, Hg2+, and Cu2+ showed strong inhibition on soybean urease, with the silver ion being a potent inhibitor (IC50 = 2.3 x 10(-8) mM).Time-dependent inhibition studies exhibited biphasic kinetics with all heavy metal ions. Furthermore, inhibition studies with sodium salts of mineral acids (NaF, NaCl, NaNO3, and Na2SO4) showed that only F- inhibited soybean urease significantly (IC50 = 2.9 mM). Competitive type of inhibition was observed for this anion with a K-i value of 1.30 mM.
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收藏
页码：646 / 652
页数：7
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