Expression and Function of the ID1 Gene During Transforming Growth Factor-β1-Induced Differentiation of Human Embryonic Stem Cells to Endothelial Cells

被引：6
作者：
Huan, Qing [1 ]
Wang, Yan [2 ]
Yang, Lixia [1 ]
Cui, Yuqian [3 ,4 ,5 ,6 ]
Wen, Ji [1 ]
Chen, Jun [1 ]
Chen, Zi-Jiang [3 ,4 ,5 ,6 ,7 ]
机构：
[1] Shandong Univ Tradit Chinese Med, Hosp 2, Reprod Med Ctr, Jinan 250001, Peoples R China
[2] Shandong Univ, Qianfoshan Hosp, Dept Plast Surg, Jinan 250021, Peoples R China
[3] Shandong Univ, Prov Hosp, Ctr Reprod Med, Jinan 250021, Peoples R China
[4] Natl Res Ctr Assisted Reprod Technol & Reprod Gen, Jinan, Peoples R China
[5] Minist Educ, Key Lab Reprod Endocrinol, Jinan, Peoples R China
[6] Shandong Prov Key Lab Reprod Med, Jinan, Peoples R China
[7] Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Shanghai 200030, Peoples R China
来源：
CELLULAR REPROGRAMMING | 2015年 / 17卷 / 01期
基金：
中国国家自然科学基金;
关键词：
LOOP-HELIX PROTEINS; IN-VITRO MODEL; TRANSCRIPTIONAL REPRESSION; CELLULAR SENESCENCE; ANGIOGENESIS; BETA; ID-1; VASCULOGENESIS; KERATINOCYTES; ACTIVATION;
D O I：
10.1089/cell.2014.0020
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
ID1 can mediate transforming growth factor-beta (TGF-beta)/activin receptor-like kinase-1 (ALK1)-induced (and Smad-dependent) migration in endothelial cells (ECs). However, the role that ID1 plays during differentiation of human embryonic stem cells (hESCs) into ECs induced by TGF-beta 1 remains unclear. In this study, a hESC differentiation model that recapitulates the developmental steps of vasculogenesis during the early stages of embryonic development was used to explore this question. We found that TGF-beta 1 increases endothelial cell differentiation and inhibits endothelial tube formation. Furthermore, at an early stage of differentiation, TGF-beta 1 may induce in vitro differentiation of hESCs into ECs by inhibiting expression of ID1, while at a later stage of differentiation, TGF-beta 1 may stimulate the proliferation and migration of ECs via the ALK1/Smad1/5/ID1 pathway. Downregulation of ID1 by gene silencing can lead to acceleration of TGF-beta 1-induced hESC differentiation into ECs and inhibition of proliferation and migration of ECs. This study may reveal some mechanisms of in vivo vasculogenesis in the early stages of embryonic development.
引用
收藏
页码：59 / 68
页数：10
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