Multifocus structured illumination microscopy for fast volumetric super-resolution imaging

被引：40

作者：

Abrahamsson, Sara ^{[1
]}

Blom, Hans ^{[2
]}

Agostinho, Ana ^{[3
]}

Jans, Daniel C. ^{[2
]}

Jost, Aurelie ^{[4
,5
,6
]}

Mueller, Marcel ^{[7
]}

Nilsson, Linnea ^{[2
]}

Bernhem, Kristoffer ^{[2
]}

Lambert, Talley J. ^{[8
]}

Heintzmann, Rainer ^{[4
,5
,6
]}

Brismar, Hjalmar ^{[2
]}

机构：

[1] Rockefeller Univ, Lulu & Anthony Wang Lab Neural Circuits & Behav, New York, NY 10021 USA

[2] KTH Royal Inst Technol, Dept Appl Phys, Sci Life Lab, Stockholm, Sweden

[3] Karolinska Inst, Dept Cell & Mol Biol, Stockholm, Sweden

[4] Friedrich Schiller Univ, Inst Phys Chem, Jena, Germany

[5] Friedrich Schiller Univ, Abbe Ctr Photon, Jena, Germany

[6] Leibniz Inst Photon Technol, Jena, Germany

[7] Bielefeld Univ, Dept Phys, Biomol Photon, Bielefeld, Germany

[8] Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USA

来源：

BIOMEDICAL OPTICS EXPRESS | 2017年 / 8卷 / 09期

关键词：

Fluorescence microscopy; Imaging systems; Microscopy; Superresolution; Three-dimensional microscopy;

D O I：

10.1364/BOE.8.004135

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We here report for the first time the synergistic implementation of structured illumination microscopy (SIM) and multifocus microscopy (MFM). This imaging modality is designed to alleviate the problem of insufficient volumetric acquisition speed in superresolution biological imaging. SIM is a wide-field super-resolution technique that allows imaging with visible light beyond the classical diffraction limit. Employing multifocus diffractive optics we obtain simultaneous wide-field 3D imaging capability in the SIM acquisition sequence, improving volumetric acquisition speed by an order of magnitude. Imaging performance is demonstrated on biological specimens. (C) 2017 Optical Society of America

引用

页码：4135 / 4140

页数：6

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