Single-molecule approaches to characterizing kinetics of biomolecular interactions

被引:90
作者
van Oijen, Antoine M. [1 ]
机构
[1] Univ Groningen, Ctr Synthet Biol, Zernike Inst Adv Mat, NL-9747 AG Groningen, Netherlands
关键词
FLUORESCENCE CORRELATION SPECTROSCOPY; CONFORMATIONAL DYNAMICS; ENZYME MOLECULES; PROTEIN INTERACTIONS; COMPLEXIN BINDING; SNARE COMPLEX; CELL BIOLOGY; MICROSCOPY; DISORDER; FRET;
D O I
10.1016/j.copbio.2010.10.002
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Single-molecule fluorescence techniques have emerged as powerful tools to study biological processes at the molecular level. This review describes the application of these methods to the characterization of the kinetics of interaction between biomolocules. A large number of single-molecule assays have been developed that visualize association and dissociation kinetics in vitro by fluorescently labeling binding partners and observing their interactions over time. Even though recent progress has been significant, there are certain limitations to this approach. To allow the observation of individual, fluorescently labeled molecules requires low, nanomolar concentrations. I will discuss how such concentration requirements in single-molecule experiments limit their applicability to investigate intermolecular interactions and how recent technical advances deal with this issue.
引用
收藏
页码:75 / 80
页数:6
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