Identification and functional characterization of a poly(A)-binding protein from Leishmania infantum (LiPABP)

被引：7
|
作者：
Guerra, Natalia [1 ]
Vega-Sendino, Maria [2 ]
Isabel Perez-Morgado, M. [1 ]
Ramos, Edurne [1 ]
Soto, Manuel [2 ]
Gonzalez, Victor M. [1 ]
Elena Martin, M. [1 ]
机构：
[1] Hosp Ramon & Cajal, Serv Bioquim Invest, IRyCIS, E-28034 Madrid, Spain
[2] Univ Autonoma Madrid, CSIC, Ctr Biol Mol Severo Ochoa, Dept Biol Mol, E-28049 Madrid, Spain
来源：
FEBS LETTERS | 2011年 / 585卷 / 01期
关键词：
Poly(A)-binding protein; Translation regulation; Leishmania infantum; POLY(A) BINDING-PROTEIN; MESSENGER-RNA; TRYPANOSOMA-BRUCEI; DOMAIN; RECOGNITION; EXPRESSION; ERF3/GSPT; CRUZI;
D O I：
10.1016/j.febslet.2010.11.042
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Gene expression regulation in Leishmania has been related to post-transcriptional events involving mainly sequences present in the 5' and 3' untranslated regions. PABPs are high-affinity poly(A)-binding proteins that are implicated in the regulation of translation initiation, RNA stability and other important biological processes. We describe a PABP from Leishmania infantum (LiPABP) that shows a very high homology with PABPs from other eukaryotic organisms, including mammals and other parasites. LiPABP conserves the main domains present in other PABPs, maintains poly(A)-binding properties and is phosphorylated by p38 mitogen-activated protein kinase. Using the sera from dogs infected with L. infantum, we demonstrate that LiPABP is expressed in L. infantum promastigotes. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
引用
收藏
页码：193 / 198
页数：6
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