Electrochemical investigations into enzymatic polymerisation of 1,10-phenanthroline-5,6-dione as a redox mediator for lactate sensing

被引：9

作者：

Halpin, Grace ^{[1
]}

Herdman, Karen ^{[1
]}

Dempsey, Eithne ^{[1
]}

机构：

[1] Maynooth Univ, Kathleen Lonsdale Inst Human Hlth, Dept Chem, Maynooth Co, Kildare, Ireland

来源：

SENSORS AND ACTUATORS REPORTS | 2021年 / 3卷

关键词：

Lactic acid; Lactate oxidase; 1,10-Phenanthroline-5,6-dione; Enzymatic polymerisation; Scanning electrochemical microscopy; LACTIC-ACID; MICROSCOPY; BIOSENSOR; OXIDASE;

D O I：

10.1016/j.snr.2021.100032

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lactate biosensor fabrication using a chitosan and crosslinker configuration realised solution mediation (K3Fe(CN)(6)) with lactate linear range 9.9 x 10(-4) to 5.66x10(-3) M, sensitivity 1.44x10(-3)C cm(-2) mM(-1) and LOD of 0.54 mM. Further development involved use of the heterocyclic quinoid species 1,10-phenanthroline-5,6-dione which acted as a proton and electron acceptor in relation to FADH(2) cofactor regeneration. Graphite ink was formulated and utilised as an underlying conductive layer for LOx enzyme immobilisation and enzymatic polymerisation of 1,10-phenanthroline-5,6-dione at a GC electrode, resulting in a linear range of 0.74 - 2.44x10(-3) M, sensitivity of 4.11x10(-4)C cm(-2) mM(-1) and LOD of 0.06 mM. Scanning electron and electrochemical scanning microscopy realised surface characterisation of the enzyme layers.

引用

页数：11

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