Discrimination of two amino acid transport activities in 4F2 heavy expressing Xenopus laevis oocytes

被引：28

作者：

Bröer, A ^{[1
]}

Hamprecht, B ^{[1
]}

Bröer, S ^{[1
]}

机构：

[1] Univ Tubingen, Inst Phys Chem, D-72076 Tubingen, Germany

来源：

BIOCHEMICAL JOURNAL | 1998年 / 333卷

关键词：

D O I：

10.1042/bj3330549

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expression of the type II membrane proteins of the rbAT/4F2hc family in Xenopus laevis oocytes results in the induction of amino acid transport activity. To elucidate the mechanism of action, amino acid transport was investigated in oocytes expressing the surface antigen 4F2hc. Leucine transport was mediated by a Na+-independent and a Na+-dependent transport mechanism. Both systems could be further discriminated by their stereochemical constraints. Isoleucine, with a branch at the beta-position, shared only the Na+-independent transport system with leucine. Both transport systems were sensitive to inhibition by arginine, but only the Na+-independent system was sensitive to inhibition by 2-aminobicyclo[2,2, 1]heptane-2-carboxylic acid. When compared with known transport systems the two transport activities could be described as similar to, but not identical with, mammalian systems b(0,+) and y(+)L. The Na+-independent b(0,+)-like transport system was found both in rbAT and 4F2hc expressing oocytes, indicating that both proteins act in a similar way.

引用

页码：549 / 554

页数：6

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