Purification and characterization of Kunitz trypsin inhibitor from soybean

被引：0

作者：

Gu, Chunmei ^{[1
]}

Li, Shujun ^{[1
]}

Song, Xinxiu ^{[1
]}

机构：

[1] Jilin Agr Univ, Inst Food Sci & Engn, Changchun 130118, Jilin, Peoples R China

来源：

PROCEEDINGS OF THE 2017 6TH INTERNATIONAL CONFERENCE ON ENERGY, ENVIRONMENT AND SUSTAINABLE DEVELOPMENT (ICEESD 2017) | 2017年 / 129卷

基金：

中国国家自然科学基金;

关键词：

soybean; Kunitz trypisin inhibitor; purification; characterization; SEEDS;

D O I：

暂无

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

In this paper, a Kunitz trypsin inhibitor from soybean meal was isolated to apparent homogeneity by a combination of phosphoric acid extraction, heat treatment, ammonium sulfate precipitation, ion exchange chromatography, affinity chromatography and gel filtration. The results showed that the specific activity of 4733 U mg(-1) and purification fold of 72.39 were obtained. The purified Kunitz trypsin inhibitor appeared a single protein band in SDS-PAGE electrophoresis. The accurate molecular mass of this inhibitor was determined as 22907.51Da by MALDI-TOF. Partial amino acid sequence of the purified protein from Edman degration showed a high degree of homology with various members of the Kunitz-inhibitor family.

引用

页码：659 / 666

页数：8

共 50 条

[41] Development of Breeder-Friendly KASP Markers for Low Concentration of Kunitz Trypsin Inhibitor in Soybean Seeds [J].

Rosso, M. Luciana ;

Shang, Chao ;

Song, Qijian ;

Escamilla, Diana ;

Gillenwater, Jay ;

Zhang, Bo .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (05) :1-16

[42] BIOCHEMICAL-CHARACTERIZATION OF A LOW TRYPSIN-INHIBITOR SOYBEAN [J].

MCNIVEN, MA ;

GRIMMELT, B ;

MACLEOD, JA ;

VOLDENG, H .

JOURNAL OF FOOD SCIENCE, 1992, 57 (06) :1375-&

[43] Exploiting the biological roles of the trypsin inhibitor from Inga vera seeds: A multifunctional Kunitz inhibitor [J].

Bezerra, Cezar da Silva ;

Ramalho de Oliveira, Caio Fernando ;

Tavares Machado, Olga Lima ;

Vieira de Mello, Gabriela Souto ;

da Rocha Pitta, Maira Galdino ;

Barreto de Melo Rego, Moacyr Jesus ;

Napoleao, Thiago Henrique ;

Guedes Paiva, Patricia Maria ;

Ferreira Ribeiro, Suzanna de Fatima ;

Gomes, Valdirene Moreira ;

Silva, Osmar Nascimento ;

Maria-Neto, Simone ;

Franco, Octavio Luiz ;

Rodrigues Macedo, Maria Ligia .

PROCESS BIOCHEMISTRY, 2016, 51 (06) :792-803

[44] A new Kunitz trypsin inhibitor from Erythrina poeppigiana exhibits antimicrobial and antibiofilm properties against bacteria [J].

Alencar de Barros, Karina Margareti ;

Orlandi Sardi, Janaina de Cassia ;

Maria-Neto, Simone ;

Macedo, Alexandre Jose ;

Ramalho, Suellen Rodrigues ;

Lourenco de Oliveira, Daniella Gorete ;

Pontes, Gemilson Soares ;

Weber, Simone Schneider ;

Ramalho de Oliveira, Caio Fernando ;

Rodrigues Macedo, Maria Ligia .

BIOMEDICINE & PHARMACOTHERAPY, 2021, 144

[45] Purification and characterization of a Kunitz inhibitor from Poincianella pyramidalis with insecticide activity against the Mediterranean flour moth [J].

Guimaraes, Lays Cordeiro ;

Ramalho de Oliveira, Caio Fernando ;

Marangoni, Sergio ;

Lourenco de Oliveira, Daniella Gorete ;

Rodrigues Macedo, Maria Ligia .

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2015, 118 :1-9

[46] Trypsin Inhibitor Isolated From Glycine max (Soya Bean) Extraction, Purification, and Characterization [J].

Khan, Sabir ;

Arshad, Shafia ;

Arif, Amina ;

Tanveer, Rida ;

Amin, Zeemal Seemab ;

Abbas, Saba ;

Maqsood, Amna ;

Raza, Muhammad ;

Munir, Arooj ;

Latif, Amara ;

Habiba, Maryam ;

Afzal, Muhammad .

DOSE-RESPONSE, 2022, 20 (04)

[47] Identification and Characterization of a Trypsin Inhibitor from Fagopyrum tataricumSeeds [J].

Ruan, Jing-Jun ;

Zhou, Mei-Liang ;

Chen, Hui ;

Shao, Ji-Rong .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2024, 196 (06) :1-17

[48] Specific-Immuno-(Chymo)Trypsin-Inhibitor-Assays for determination of (residual) activity of Bowman-Birk or Kunitz Soybean Trypsin Inhibitors [J].

van Amerongen, A ;

Ostafe, V ;

Meijer, MMT ;

Gruppen, H ;

Meerdink, G ;

Berendsen, LBJM ;

Koets, M ;

Wichers, JH .

RECENT ADVANCES OF RESEARCH IN ANTINUTRITIONAL FACTORS IN LEGUME SEEDS AND RAPESEED, 1998, (93) :33-37

[49] Bioinsecticidal activity of a novel Kunitz trypsin inhibitor from Catanduva (Piptadenia moniliformis) seeds [J].

Cruz, Ana C. B. ;

Massena, Fabio S. ;

Migliolo, Ludovico ;

Macedo, Leonardo L. P. ;

Monteiro, Norberto K. V. ;

Oliveira, Adeliana S. ;

Macedo, Francisco P. ;

Uchoa, Adriana F. ;

Grossi de Sa, Maria F. ;

Vasconcelos, Ilka M. ;

Murad, Andre M. ;

Franco, Octavio L. ;

Santos, Elizeu A. .

PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2013, 70 :61-68

[50] Primary structure of a Kunitz-type trypsin inhibitor from Enterolobium contortisiliquum seeds [J].

Batista, IFC ;

Oliva, MLV ;

Araujo, MS ;

Sampaio, MU ;

Richardson, M ;

Fritz, H ;

Sampaio, CAM .

PHYTOCHEMISTRY, 1996, 41 (04) :1017-1022

← 1 2 3 4 5 →