Purification and characterization of Kunitz trypsin inhibitor from soybean

被引：0

作者：

Gu, Chunmei ^{[1
]}

Li, Shujun ^{[1
]}

Song, Xinxiu ^{[1
]}

机构：

[1] Jilin Agr Univ, Inst Food Sci & Engn, Changchun 130118, Jilin, Peoples R China

来源：

PROCEEDINGS OF THE 2017 6TH INTERNATIONAL CONFERENCE ON ENERGY, ENVIRONMENT AND SUSTAINABLE DEVELOPMENT (ICEESD 2017) | 2017年 / 129卷

基金：

中国国家自然科学基金;

关键词：

soybean; Kunitz trypisin inhibitor; purification; characterization; SEEDS;

D O I：

暂无

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

In this paper, a Kunitz trypsin inhibitor from soybean meal was isolated to apparent homogeneity by a combination of phosphoric acid extraction, heat treatment, ammonium sulfate precipitation, ion exchange chromatography, affinity chromatography and gel filtration. The results showed that the specific activity of 4733 U mg(-1) and purification fold of 72.39 were obtained. The purified Kunitz trypsin inhibitor appeared a single protein band in SDS-PAGE electrophoresis. The accurate molecular mass of this inhibitor was determined as 22907.51Da by MALDI-TOF. Partial amino acid sequence of the purified protein from Edman degration showed a high degree of homology with various members of the Kunitz-inhibitor family.

引用

页码：659 / 666

页数：8

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