Purification and characterization of Kunitz trypsin inhibitor from soybean

被引：0
|
作者：
Gu, Chunmei [1 ]
Li, Shujun [1 ]
Song, Xinxiu [1 ]
机构：
[1] Jilin Agr Univ, Inst Food Sci & Engn, Changchun 130118, Jilin, Peoples R China
来源：
PROCEEDINGS OF THE 2017 6TH INTERNATIONAL CONFERENCE ON ENERGY, ENVIRONMENT AND SUSTAINABLE DEVELOPMENT (ICEESD 2017) | 2017年 / 129卷
基金：
中国国家自然科学基金;
关键词：
soybean; Kunitz trypisin inhibitor; purification; characterization; SEEDS;
D O I：
暂无
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
In this paper, a Kunitz trypsin inhibitor from soybean meal was isolated to apparent homogeneity by a combination of phosphoric acid extraction, heat treatment, ammonium sulfate precipitation, ion exchange chromatography, affinity chromatography and gel filtration. The results showed that the specific activity of 4733 U mg(-1) and purification fold of 72.39 were obtained. The purified Kunitz trypsin inhibitor appeared a single protein band in SDS-PAGE electrophoresis. The accurate molecular mass of this inhibitor was determined as 22907.51Da by MALDI-TOF. Partial amino acid sequence of the purified protein from Edman degration showed a high degree of homology with various members of the Kunitz-inhibitor family.
引用
收藏
页码：659 / 666
页数：8
相关论文
共 50 条
  • [1] One-step purification of Kunitz soybean trypsin inhibitor
    Duranti, M
    Barbiroli, A
    Scaraforti, A
    Tedeschi, G
    Morazzoni, P
    PROTEIN EXPRESSION AND PURIFICATION, 2003, 30 (02) : 167 - 170
  • [2] Genetic characterization of a novel soybean Kunitz trypsin inhibitor
    Wang, KJ
    Takahata, Y
    Ito, K
    Zhao, YP
    Tsutsumi, K
    Kaizuma, N
    BREEDING SCIENCE, 2001, 51 (03) : 185 - 190
  • [3] SOYBEAN TRYPSIN-INHIBITOR (KUNITZ) IS A DOUBLEHEADED INHIBITOR
    QUAST, U
    STEFFEN, E
    HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1975, 356 (05): : 617 - 620
  • [4] Reversible denaturation of the soybean Kunitz trypsin inhibitor
    Roychaudhuri, R
    Sarath, G
    Zeece, M
    Markwell, J
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2003, 412 (01) : 20 - 26
  • [5] Stability of the allergenic soybean Kunitz trypsin inhibitor
    Roychaudhuri, R
    Sarath, G
    Zeece, M
    Markwell, J
    BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2004, 1699 (1-2): : 207 - 212
  • [6] Purification of lectin and Kunitz trypsin inhibitor from soya seeds
    Roy, Uttam K.
    Lavignac, Nathalie
    Rahman, Azizur M.
    Nielsen, Birthe V.
    JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2018, 56 (05) : 436 - 442
  • [7] ISOLATION AND CHARACTERIZATION OF GENETIC-VARIANTS OF THE KUNITZ SOYBEAN TRYPSIN-INHIBITOR
    FREED, RC
    RYAN, DS
    BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 624 (02) : 562 - 572
  • [8] Purification and Characterization of a Kunitz-Type Trypsin Inhibitor from Acacia victoriae Bentham Seeds
    Ee, Kah Y.
    Zhao, Jian
    Rehman, Ata-Ur
    Agboola, Samson D.
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2009, 57 (15) : 7022 - 7029
  • [9] Purification of soybean Kunitz trypsin inhibitor and the mechanism of its passivation by lysine and disulfide bond modifications
    Kong, Xin
    Li, You
    Liu, Xinqi
    FOOD BIOSCIENCE, 2023, 55
  • [10] Purification and Quantification of Kunitz Trypsin Inhibitor in Soybean Using Two-Dimensional Liquid Chromatography
    Zhou, Tianjiao
    Han, Shuaijuan
    Li, Zhen
    He, Pingli
    FOOD ANALYTICAL METHODS, 2017, 10 (10) : 3350 - 3360
12345