Intravital Microscopic Interrogation of Peripheral Taste Sensation

被引：17

作者：

Choi, Myunghwan ^{[1
,2
,3
]}

Lee, Woei Ming ^{[1
,2
,4
]}

Yun, Seok Hyun ^{[1
,2
]}

机构：

[1] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Cambridge, MA 02139 USA

[2] Massachusetts Gen Hosp, Wellman Ctr Photomed, Cambridge, MA 02139 USA

[3] Sungkyunkwan Univ, Global Biomed Engn, Suwon, South Korea

[4] Australian Natl Univ, Res Sch Engn, Canberra, ACT 0200, Australia

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

新加坡国家研究基金会; 美国国家卫生研究院;

关键词：

SWEET TASTE; RECEPTORS; TONGUE; CELLS; BUDS;

D O I：

10.1038/srep08661

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Intravital microscopy is a powerful tool in neuroscience but has not been adapted to the taste sensory organ due to anatomical constraint. Here we developed an imaging window to facilitate microscopic access to the murine tongue in vivo. Real-time two-photon microscopy allowed the visualization of three-dimensional microanatomy of the intact tongue mucosa and functional activity of taste cells in response to topically administered tastants in live mice. Video microscopy also showed the calcium activity of taste cells elicited by small-sized tastants in the blood circulation. Molecular kinetic analysis suggested that intravascular taste sensation takes place at the microvilli on the apical side of taste cells after diffusion of the molecules through the pericellular capillaries and tight junctions in the taste bud. Our results demonstrate the capabilities and utilities of the new tool for taste research in vivo.

引用

页数：6

共 33 条

[31] Optogenetics in Neural Systems [J].