Bioluminescence assays: multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay

被引：50

作者：

Nakajima, Yoshihiro ^{[2
]}

Ohmiya, Yoshihiro ^{[1
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Tsukuba, Ibaraki, Japan

[2] Natl Inst Adv Ind Sci & Technol, Hlth Res Inst, Ikeda, Osaka, Japan

来源：

EXPERT OPINION ON DRUG DISCOVERY | 2010年 / 5卷 / 09期

基金：

日本学术振兴会;

关键词：

beetle luciferase; bioluminescence; bioluminescence imaging; color differenced luciferases; reporter assay; reporter gene; secreted luciferase; IN-VIVO; FIREFLY LUCIFERASE; REPORTER ASSAY; CIRCADIAN-RHYTHMS; GENE TECHNOLOGY; CDNA CLONING; EXPRESSION; RED; SYSTEM; CELLS;

D O I：

10.1517/17460441.2010.506213

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Importance of the field: By selecting the most appropriate bioluminescence assay, the researcher can study the underlying molecular mechanisms of a physiological system and the effects of a drug throughout the body. Areas covered in this review: This review covers three luciferase assay systems: the multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay. These assays are applied to drug screening in vitro, in cellulo and in vivo. What the reader will gain: Different solutions for reporter assay in vitro, in cellulo and in vivo are presented. A suitable bioluminescence system depending on the assay purpose is also discussed. Take home message: Bioluminescence is a manifold system based on the different types of luciferin and its luciferase. Namely, luciferin catalyzed by corresponding luciferases resulted in the production of different color lights. We must understand the manifold mechanisms of bioluminescence reaction.

引用

页码：835 / 849

页数：15

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