Bioluminescence assays: multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay

被引：50
作者：
Nakajima, Yoshihiro [2 ]
Ohmiya, Yoshihiro [1 ]
机构：
[1] Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Tsukuba, Ibaraki, Japan
[2] Natl Inst Adv Ind Sci & Technol, Hlth Res Inst, Ikeda, Osaka, Japan
来源：
EXPERT OPINION ON DRUG DISCOVERY | 2010年 / 5卷 / 09期
基金：
日本学术振兴会;
关键词：
beetle luciferase; bioluminescence; bioluminescence imaging; color differenced luciferases; reporter assay; reporter gene; secreted luciferase; IN-VIVO; FIREFLY LUCIFERASE; REPORTER ASSAY; CIRCADIAN-RHYTHMS; GENE TECHNOLOGY; CDNA CLONING; EXPRESSION; RED; SYSTEM; CELLS;
D O I：
10.1517/17460441.2010.506213
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Importance of the field: By selecting the most appropriate bioluminescence assay, the researcher can study the underlying molecular mechanisms of a physiological system and the effects of a drug throughout the body. Areas covered in this review: This review covers three luciferase assay systems: the multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay. These assays are applied to drug screening in vitro, in cellulo and in vivo. What the reader will gain: Different solutions for reporter assay in vitro, in cellulo and in vivo are presented. A suitable bioluminescence system depending on the assay purpose is also discussed. Take home message: Bioluminescence is a manifold system based on the different types of luciferin and its luciferase. Namely, luciferin catalyzed by corresponding luciferases resulted in the production of different color lights. We must understand the manifold mechanisms of bioluminescence reaction.
引用
收藏
页码：835 / 849
页数：15
相关论文
共 87 条
[41]   Spectral-resolved gene technology for multiplexed bioluminescence and high-content screening [J].
Michelini, Elisa ;
Cevenini, Luca ;
Mezzanotte, Laura ;
Ablamsky, Danielle ;
Southworth, Tara ;
Branchini, Bruce ;
Roda, Aldo .
ANALYTICAL CHEMISTRY, 2008, 80 (01) :260-267
[42]   Resilient circadian oscillator revealed in individual cyanobacteria [J].
Mihalcescu, I ;
Hsing, WH ;
Leibler, S .
NATURE, 2004, 430 (6995) :81-85
[43]   Real-time imaging of ß-catenin dynamics in cells and living mice [J].
Naik, Snehal ;
Piwnica-Worms, David .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (44) :17465-17470
[44]   Multicolor luciferase assay system: one-step monitoring of multiple gene expressions with a single substrate [J].
Nakajima, Y ;
Kimura, T ;
Sugata, K ;
Enomoto, T ;
Asakawa, A ;
Kubota, H ;
Ikeda, M ;
Ohmiya, Y .
BIOTECHNIQUES, 2005, 38 (06) :891-894
[45]   Bidirectional role of orphan nuclear receptor RORα in clock gene transcriptions demonstrated by a novel reporter assay system [J].
Nakajima, Y ;
Ikeda, M ;
Kimura, T ;
Honma, S ;
Ohmiya, Y ;
Honma, K .
FEBS LETTERS, 2004, 565 (1-3) :122-126
[46]   CDNA cloning and characterization of a secreted luciferase from the luminous Japanese ostracod, Cypridina noctiluca [J].
Nakajima, Y ;
Kobayashi, K ;
Yamagishi, K ;
Enomoto, T ;
Ohmiya, Y .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2004, 68 (03) :565-570
[47]   Enhanced Beetle Luciferase for High-Resolution Bioluminescence Imaging [J].
Nakajima, Yoshihiro ;
Yamazaki, Tomomi ;
Nishii, Shigeaki ;
Noguchi, Takako ;
Hoshino, Hideto ;
Niwa, Kazuki ;
Viviani, Vadim R. ;
Ohmiya, Yoshihiro .
PLOS ONE, 2010, 5 (03)
[48]   Reporter gene technology: The future looks bright [J].
Naylor, LH .
BIOCHEMICAL PHARMACOLOGY, 1999, 58 (05) :749-757
[49]   New reporter system for Per1 and Bmal1 expressions revealed self-sustained circadian rhythms in peripheral tissues [J].
Nishide, Shin-ya ;
Honma, Sato ;
Nakajima, Yoshihiro ;
Ikeda, Masaaki ;
Baba, Kenkichi ;
Ohmiya, Yoshihiro ;
Honma, Ken-ichi .
GENES TO CELLS, 2006, 11 (10) :1173-1182
[50]   Inhibitory effect of lipoic acid on firefly luciferase bioluminescence [J].
Niwa, K ;
Ohmiya, Y .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 323 (02) :625-629
123456789