Validation of the modified agglutination test for the detection of Toxoplasma gondii in free-range chickens by using cat and mouse bioassay

被引：62

作者：

Dubey, J. P. ^{[1
]}

Laurin, E. ^{[2
]}

Kwowk, O. C. H. ^{[1
]}

机构：

[1] USDA ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, BARC East,Bldg 1001, Beltsville, MD 20705 USA

[2] Atlantic Vet Coll, Dept Hlth Management, 550 Univ Ave, Charlottetown, PE C1A 4P3, Canada

来源：

PARASITOLOGY | 2016年 / 143卷 / 03期

关键词：

Toxoplasma gondii; chickens; modified agglutination test; bioassay; MOLECULAR CHARACTERIZATION; GENETIC-CHARACTERISTICS; TISSUE DISTRIBUTION; BIOLOGIC CHARACTERISTICS; UNEXPECTED FINDINGS; GALLUS-DOMESTICUS; HIGH PREVALENCE; CENTRAL-AMERICA; SOUTH-AMERICA; UNITED-STATES;

D O I：

10.1017/S0031182015001316

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The modified agglutination test (MAT) is one of the most commonly used tests for the detection of antibodies to Toxoplasma gondii in animal and human sera. The objective of the present study was to evaluate the diagnostic accuracy of the MAT and bioassay in free-range/backyard (FR) chickens (Gallus domesticus). Previously-published T. gondii test results from 2066 chickens from 19 countries were compiled for the present study. The frequency of isolation of T. gondii increased for MAT titres between 1:5 and >= 1:160, and ranged from 61 to 75% for antibody titres of 1:160, 1:320, and 1:640. Twenty-three cats fed pooled hearts from a total of 802 FR seronegative (MAT, <1:5) chickens from several countries did not excrete oocysts, indicating a high negative predictive value of MAT because FR chickens would have been exposed to many microbes; cats are the most sensitive indicators of T. gondii infection in tissues and can excrete millions of oocysts after ingesting even a few bradyzoites. Of the 29 cats in this study, six cats, fed hearts pooled from 15-122 FR chickens, excreted oocysts; but these identifications were likely related to misidentification or prozone. Results of the present study support the validity of MAT for the detection of T. gondii infection in chickens.

引用

页码：314 / 319

页数：6

共 40 条

[1] Sensitivity and specificity of serological tests, histopathology and immunohistochemistry for detection of Toxoplasma gondii infection in domestic chickens [J].

Casartelli-Alves, L. ;

Boechat, V. C. ;

Macedo-Couto, R. ;

Ferreira, L. C. ;

Nicolau, J. L. ;

Neves, L. B. ;

Millar, P. R. ;

Vicente, R. T. ;

Oliveira, R. V. C. ;

Muniz, A. G. ;

Bonna, I. C. F. ;

Amendoeira, M. R. R. ;

Silva, R. C. ;

Langoni, H. ;

Schubach, T. M. P. ;

Menezes, R. C. .

VETERINARY PARASITOLOGY, 2014, 204 (3-4) :346-351

[2]

da Silva DS, 2003, J PARASITOL, V89, P394, DOI 10.1645/0022-3395(2003)089[0394:POTGIC]2.0.CO

[3]

[4] Seroprevalence and isolation of Toxoplasma gondii from free-range chickens in Ghana, Indonesia, Italy, Poland, and Vietnam [J].

Dubey, J. P. ;

Huong, Lam Thi Thu ;

Lawson, B. W. L. ;

Subekti, D. T. ;

Tassi, P. ;

Cabaj, W. ;

Sundar, N. ;

Velmurugan, G. V. ;

Kwok, O. C. H. ;

Su, C. .

JOURNAL OF PARASITOLOGY, 2008, 94 (01) :68-71

[5] Endemic avian toxoplasmosis on a farm in Illinois:: Clinical disease, diagnosis, biologic and genetic characteristics of Toxoplasma gondii isolates from chickens (Gallus domesticus), and a goose (Anser anser) [J].

Dubey, J. P. ;

Webb, D. M. ;

Sundar, N. ;

Velmurugan, G. V. ;

Bandini, L. A. ;

Kwok, O. C. H. ;

Su, C. .

VETERINARY PARASITOLOGY, 2007, 148 (3-4) :207-212

[6] Biologic and genetic characteristics of Toxoplasma gondii isolates in free-range chickens from Nicaragua, Central America [J].

Dubey, J. P. ;

Sundar, N. ;

Pineda, N. ;

Kyvsgaard, N. C. ;

Luna, L. A. ;

Rimbaud, E. ;

Oliveira, J. B. ;

Kwok, O. C. H. ;

Qi, Y. ;

Su, C. .

VETERINARY PARASITOLOGY, 2006, 142 (1-2) :47-53

[7] Characterization of Toxoplasma gondii isolates in free-range chickens from Chile, South America [J].

Dubey, J. P. ;

Patitucci, A. N. ;

Su, C. ;

Sundar, N. ;

Kwok, O. C. H. ;

Shen, S. K. .

VETERINARY PARASITOLOGY, 2006, 140 (1-2) :76-82

[8] Toxoplasmosis in humans and animals in Brazil: high prevalence, high burden of disease, and epidemiology [J].

Dubey, J. P. ;

Lago, E. G. ;

Gennari, S. M. ;

Su, C. ;

Jones, J. L. .

PARASITOLOGY, 2012, 139 (11) :1375-1424

[9] NEW TOXOPLASMA GONDII GENOTYPES ISOLATED FROM FREE-RANGE CHICKENS FROM THE FERNANDO DE NORONHA, BRAZIL: UNEXPECTED FINDINGS [J].

Dubey, J. P. ;

Rajendran, C. ;

Costa, D. G. C. ;

Ferreira, L. R. ;

Kwok, O. C. H. ;

Qu, D. ;

Su, C. ;

Marvulo, M. F. V. ;

Alves, L. C. ;

Mota, R. A. ;

Silva, J. C. R. .

JOURNAL OF PARASITOLOGY, 2010, 96 (04) :709-712

[10] Toxoplasma gondii Infections in Chickens (Gallus domesticus): Prevalence, Clinical Disease, Diagnosis and Public Health Significance [J].

Dubey, J. P. .

ZOONOSES AND PUBLIC HEALTH, 2010, 57 (01) :60-73

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