Performance Evaluation of Serum Free Light Chain Analysis Nephelometry vs Turbidimetry, Monoclonal vs Polyclonal Reagents

被引：0

作者：

Messiaen, Anne-Sophie ^{[1
]}

De Sloovere, Maxime M. W. ^{[1
]}

Claus, Paul-Emile ^{[1
]}

Vercammen, Martine ^{[2
]}

Van Hoovels, Lieve ^{[3
]}

Heylen, Olivier ^{[4
]}

Debrabandere, Johan ^{[1
]}

Vanpoucke, Hilde ^{[1
]}

De Smet, Dieter ^{[1
]}

机构：

[1] AZ Delta Roeselare Menen, Dept Lab Med, Ardooisesteenweg 276, B-8800 Roeselare, Belgium

[2] AZ Sint Jan Brugge Oostende, Dept Lab Med, Brugge, Belgium

[3] OLV Aalst Asse, Dept Lab Med, Aalst, Belgium

[4] AZ Groeninge, Dept Lab Med, Kortrijk, Belgium

来源：

AMERICAN JOURNAL OF CLINICAL PATHOLOGY | 2017年 / 147卷 / 06期

关键词：

Free light chains; Monoclonal; Polyclonal; Nephelometry; Turbidimetry; Myeloma; Monoclonal gammopathy;

D O I：

10.1093/AJCP/AQX037

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Objectives: Free light chain (FLC) measurement gained a lot of interest for diagnostic workup of monoclonal gammopathy. Methods: We evaluated the performance of turbidimetric polyclonal Freelite (The Binding Site, Birmingham, UK) assays on Cobas 6000 (Roche Diagnostics, Rotkreuz, Switzerland) and nephelometric monoclonal N Latex (Siemens Healthcare Diagnostics, Marburg, Germany) assays on BN ProSpec (Dade Behring, Deerfield, IL) vs established nephelometric Freelite assays on BN ProSpec. Results: Analytical performance was acceptable. Method comparison (n = 118) showed significant proportional FLC differences for N Latex assays. However, good correlation and clinical concordance were shown. Recovery study in the low concentration range demonstrated consistent over-and underrecovery for Freelite reagents, hampering future research on prognostic value of suppressed noninvolved FLC. Antigen excess detection was successful for. FLC in three-fourths of cases with Freelite reagents and in all cases with N Latex reagents. However, the latter resulted in underestimated. FLC concentrations. Conclusions: FLC analysis requires continuous awareness of analytical limitations. Monitoring of disease response requires FLC analysis on the same platform using the same reagents.

引用

页码：611 / 622

页数：12