Protein crystals orientation in a magnetic field

被引：55

作者：

Astier, JP ^{[1
]}

Veesler, S ^{[1
]}

Boistelle, R ^{[1
]}

机构：

[1] Ctr Rech Mecanismes Croissance Cristalline, CNRS, F-13288 Marseille 09, France

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1998年 / 54卷

关键词：

D O I：

10.1107/S0907444997020052

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleation and crystal growth of hen egg-white lysozyme, bovine pancreatic trypsin inhibitor and porcine pancreatic cr-amylase were carried out in the presence of a magnetic field of 1.25 T produced by small permanent magnets. Crystals were oriented in the magnetic field, except when heterogeneous nucleation occurred. The orientation of protein crystals in the presence of a magnetic field can be attributed to the anisotropic diamagnetic susceptibility of proteins resulting from the large anisotropy of the alpha-helices due to the axial alignment of the peptide bonds.

引用

页码：703 / 706

页数：4

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