The MTCY428.08 gene of Mycobacterium tuberculosis codes for NAD+ synthetase

被引：25
作者：
Cantoni, R
Branzoni, M
Labò, M
Rizzi, M
Riccardi, G
机构：
[1] Univ Pavia, Dipartimento Genet & Microbiol, I-27100 Pavia, Italy
[2] Univ Turin, Dipartimento Sci & Tecnol Farmaco, I-10125 Turin, Italy
来源：
JOURNAL OF BACTERIOLOGY | 1998年 / 180卷 / 12期
关键词：
D O I：
10.1128/JB.180.12.3218-3221.1998
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The product of the MTCY428.08 gene of Mycobacterium tuberculosis shows sequence homology with several NAD(+) synthetases. The MTCY428.08 gene was cloned into the expression vectors pGEX-4T-1 and pET-15b. Expression in Escherichia coli led to overproduction of glutathione S-transferase fused and His(6)-tagged gene products, which were enzymatically assayed for NAD synthetase activity. Our results demonstrate that the MTCP428.08 gene of M. tuberculosis is the structural gene for NAD(+) synthetase.
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收藏
页码：3218 / 3221
页数：4
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