In vivo 13C NMR assessment of brain glycogen concentration and turnover in the awake rat

被引:57
作者
Choi, IY
Gruetter, R
机构
[1] Univ Minnesota, Sch Med, Ctr Magnet Resonance Res, Dept Radiol, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Sch Med, Dept Neurosci, Minneapolis, MN 55455 USA
关键词
MRS; localization; in vivo; glycogen; NAA; brain;
D O I
10.1016/S0197-0186(03)00018-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Brain glycogen metabolism was recently observed in vivo and found to be very slow in the lightly alpha-chloralose anesthetized rat [J. Neurochem. 73 (1999) 1300]. Based on that slow turnover, the total glycogen content in the awake rat brain and its turnover time were assessed after administering C-13-labeled glucose for 48 h. Label incorporation into glycogen, glucose, amino acid, and N-acetyl-aspartate (NAA) resonances was observed. The amount of C-13 label incorporated into glycogen was variable and did not correlate with that in glutamate (r = -0.1, P > 0.86). However, the amount of C-13 label incorporated into glycogen was very similar to that in NAA (r = 0.93), implying similar turnover times between brain glycogen and NAA (similar to10h). Absolute quantification of the total concentration of brain glycogen in the awake, normoglycemic rat yielded 3.3 +/- 0.8 mumol/g (n = 6, mean +/- S.D.). (C) 2003 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:317 / 322
页数:6
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