A comparative study on the disintegration of filamentous fungi

被引：23

作者：

Taubert, J ^{[1
]}

Krings, U ^{[1
]}

Berger, RG ^{[1
]}

机构：

[1] Univ Hannover, Inst Lebensmittelchem, D-30453 Hannover, Germany

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2000年 / 42卷 / 03期

关键词：

disintegration; disruption; filamentous fungi; intracellular compounds; enzymes;

D O I：

10.1016/S0167-7012(00)00194-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Different methods for cell disintegration were tested for their efficacy on filamentous fungi, including percussion grinding, homogenization using an Ultra-Turrax, chemical treatment and lyophylization. The release of protein from Ganoderma applanatum and Pycnoporus cinnabarinus and the activity of cytoplasmatic glucose-6-phosphate dehydrogenase in the crude extracts were monitored to determine the efficiency of each disintegration technique used. Fungal cells proved to be particularly resistant towards some disintegration methods commonly used for yeasts and bacteria. Best results were obtained using a percussion grinder, if necessary, in combination with an Ultra-Turrax pretreatment. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：225 / 232

页数：8

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