Efficiency and specificity in microRNA biogenesis

被引：15

作者：

Barad, Omer ^{[1
]}

Mann, Mati ^{[1
,2
]}

Chapnik, Elik ^{[1
]}

Shenoy, Archana ^{[3
,4
]}

Blelloch, Robert ^{[3
,4
]}

Barkai, Naama ^{[1
]}

Hornstein, Eran ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel

[2] Weizmann Inst Sci, Dept Mol Cell Biol, IL-76100 Rehovot, Israel

[3] Univ Calif San Francisco, Ctr Reprod Sci, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell, San Francisco, CA 94143 USA

[4] Univ Calif San Francisco, Dept Urol, San Francisco, CA USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2012年 / 19卷 / 06期

基金：

美国国家卫生研究院; 欧洲研究理事会; 以色列科学基金会;

关键词：

MICROPROCESSOR COMPLEX; DGCR8; IDENTIFICATION; EXPRESSION; DEFICITS; TISSUES; DROSHA;

D O I：

10.1038/nsmb.2293

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Primary microRNA cleavage by the Drosha-Dgcr8 'Microprocessor' complex is critical for microRNA biogenesis. Yet, the Microprocessor may also cleave other nuclear RNAs in a nonspecific manner. We studied Microprocessor function using mathematical modeling and experiments in mouse and human tissues. We found that the autoregulatory feedback on Microprocessor expression is instrumental for balancing the efficiency and specificity of its activity by effectively tuning Microprocessor levels to those of its pri-miRNA substrate.

引用

页码：650 / +

页数：5

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