Genotoxicity and oxidative stress induced by the fungicide azoxystrobin in zebrafish (Danio rerio) livers

被引：125

作者：

Han, Yingnan ^{[1
]}

Liu, Tong ^{[1
]}

Wang, Jinhua ^{[1
]}

Wang, Jun ^{[1
]}

Zhang, Cheng ^{[1
]}

Zhu, Lusheng ^{[1
]}

机构：

[1] Shandong Agr Univ, Natl Engn Lab Efficient Utilizat Soil & Fertilize, Coll Resources & Environm, Key Lab Agr Environm Univ Shandong, Tai An 271018, Shandong, Peoples R China

来源：

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY | 2016年 / 133卷

基金：

中国国家自然科学基金;

关键词：

ROS; SOD; CAT; GST; LPO; Comet assay; DNA-DAMAGE; ANTIOXIDANT ENZYMES; LIPID-PEROXIDATION; STROBILURIN; GLUTATHIONE; EXPRESSION; EXPOSURE; FISH; QUANTITATION; RESPONSES;

D O I：

10.1016/j.pestbp.2016.03.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Azoxystrobin is a frequently used fungicide in agriculture. Its toxicological effects on non-target organisms have aroused attention. In the present work, the toxic effects of azoxystrobin on zebrafish (Danio rerio) were investigated. Male and female zebrafish were separately exposed to a control solution and three azoxystrobin treatments (1, 10, and 100 mu g/L) and were sampled on days 7, 14, 21, and 28. Reactive oxygen species (ROS) were accumulated in excess in the zebrafish livers. Superoxide dismutase (SOD) activity was significantly inhibited in the male zebrafish. Moreover, a notable decrease was also observed after day 21 in the female zebrafish. Catalase (CAT) activity was induced by the azoxystrobin treatments with the exception of the 1 mu g/L treatment. A significant increase in glutathione-S-transferase (GST) activity was observed after day 21. Lipid peroxidation (LPO) was generated, and DNA damage was enhanced in a concentration-dependent manner. In conclusion, azoxystrobin induced oxidative stress and genotoxicity in zebrafish livers. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：13 / 19

页数：7

共 45 条

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