In vitro study of the interactions of galactosylated thermo-responsive hydrogels with cells

被引：9
作者：
Yang, Ning [1 ]
Chen, Li [1 ]
Yang, Miao-kun [1 ]
Bi, Si-xin [1 ]
He, Xiao-ling [1 ]
Zhu, Zheng-yan [2 ]
Yu, Mei-li [3 ]
机构：
[1] Tianjin Polytech Univ, Tianjin Key Lab Fiber Modificat & Funct Fiber, Sch Mat Sci & Engn, Tianjin 300387, Peoples R China
[2] Tianjin Third Cent Hosp, Key Lab Regenerat Med, Tianjin 300170, Peoples R China
[3] Tianjin Third Cent Hosp, Inst Liver & Gall Dis, Tianjin 300170, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2012年 / 88卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Cell scaffolds; Thermo-responsive hydrogels; N-isopropyl acrylamide; Galactosylated acrylate; HL-7702; cells; TEMPERATURE; APOPTOSIS; SURFACE; BIOMATERIALS; POLYSTYRENE; ATTACHMENT; DETACHMENT; POLYMERS; CHITOSAN; SCAFFOLD;
D O I：
10.1016/j.carbpol.2011.12.036
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The aim of this work is to explore the ability of poly(N-isopropylacrylamide) (NIPAAm) with galactosylated acrylate (GAC) hydrogels as cell scaffolds for HL-7702 cells to proliferate, keep function of albumin and urea synthesis and detach. The results indicate poly(NIPAAm-co-GAC) hydrogels with weight ratio of 0.2 could support cells to adhere, proliferate and maintain their liver functions for 7 days. An intact cells sheet could spontaneously detach from poly(NIPAAm-co-GAC) (r = 0.2) hydrogels without treating with any enzymes. And detached cells show more integrate morphology (H&E staining) and higher proliferation index (cell cycle analysis) than cells detached by trypsin treatment. The results also reveal excessive GAC introduced to PNIPAAm hydrogels could induce apoptotic cell death of adhered cells. (c) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码：509 / 516
页数：8
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