Evaluating Noninvasive Methods of Sex Identification in Green Sea Turtle (Chelonia mydas) Hatchlings

被引：17

作者：

Xia, Zhong-Rong ^{[1
]}

Li, Pi-Peng ^{[2
]}

Gu, He-Xiang

Fong, Jonathan J. ^{[3
]}

Zhao, Er-Mi ^{[1
,4
]}

机构：

[1] Sichuan Univ, Coll Life Sci, Key Lab Bioresources & Ecoenvironm, Minist Educ, Chengdu 610064, Sichuan, Peoples R China

[2] Shenyang Normal Univ, Herpetodivers Res Grp, Shenyang 110034, Peoples R China

[3] Univ Calif Berkeley, Museum Vertebrate Zool, Dept Integrat Biol, Berkeley, CA 94720 USA

[4] Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China

来源：

CHELONIAN CONSERVATION AND BIOLOGY | 2011年 / 10卷 / 01期

关键词：

temperature sex determination; Estradiol; Testosterone; Guangdong Province; Sea Turtle Bay; CARETTA-CARETTA; RATIO; DIFFERENTIATION; CONSERVATION; TEMPERATURES; ESTRADIOL; PERIOD;

D O I：

10.2744/CCB-0852.1

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

The goal of this study was to test the effectiveness of analyzing sex hormone profiles to sex green sea turtle (Chelonia mydas) hatchlings. To assess the efficacy of this method, 200 eggs of C. mydas were incubated at five different temperatures, and a chemiluminescent immunoassay system was used to determine the sex hormone profiles (estrogen and testosterone) in the amniotic fluid (from eggshells) and plasma of a subset of C. mydas hatchlings. Results were compared to a standard histological technique and revealed that evaluating sex hormone profiles from amniotic fluid is an effective, noninvasive technique for determining hatchling gender.

引用

页码：117 / 123

页数：7

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