Integrin linked kinase as a candidate downstream effector in proteinuria

被引:94
作者
Kretzler, M
Teixeira, VPC
Unschuld, PG
Cohen, CD
Wanke, R
Edenhofer, I
Mundel, P
Schlöndorff, D
Holthöfer, H
机构
[1] Univ Munich, Med Poliklin, D-80336 Munich, Germany
[2] Univ Munich, Inst Vet Pathol, D-80336 Munich, Germany
[3] Albert Einstein Coll Med, Dept Anat & Cell Biol, New York, NY USA
[4] Univ Helsinki, Dept Immunol, FIN-00014 Helsinki, Finland
关键词
glomerulus; nephrotic syndrome; actin; actinin; extracellular matrix;
D O I
10.1096/fj.00-0832fje
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The kidney glomerulus is responsible for the generation of a protein-free ultrafiltrate. After injury, it shows a characteristic, uniform response leading to progressive renal failure. Recent studies of hereditary proteinuric diseases have revealed mutations in molecules involved in podocyte cell-cell and cell-matrix interaction. Associated cellular signaling events activated in proteinuria have not been analyzed so far. To identify proteinuria-induced molecules, we used mRNA differential display in glomeruli from children with congenital nephrotic syndrome of the Finnish type. An increase in integrin linked kinase (ILK) mRNA, beta (1)-integrin coupled serine threonine kinase, was identified. Observations suggest a role for ILK in glomerular failure. An up-regulation of glomerular and Single-podocyte ILK mRNA was found in two murine models of proteinuria. In cultured podocytes, integrin attachment to matrix inhibited ILK activity and podocyte damage with puromycin activated ILK. Stable overexpression of ILK in murine podocytes caused reduced matrix adhesion and led to considerable phenotype alteration compared with expression of a kinase-defective ILK, paralleling aspects of in vivo podocyte damage. These results are consistent with ILK as a relevant player in the orchestration of podocyte matrix interaction and a candidate downstream regulator of podocyte permselectivity in glomerular diseases.
引用
收藏
页码:1843 / +
页数:22
相关论文
共 36 条
[1]  
Adler S, 1996, AM J PATHOL, V149, P987
[2]  
ADLER S, 1992, AM J PATHOL, V141, P571
[3]  
ADLER S, 1994, KIDNEY INT S, V45, P86
[4]   Cloning and expression of the rat nephrin homolog [J].
Ahola, H ;
Wang, SX ;
Luimula, P ;
Solin, ML ;
Holzman, LB ;
Holthöfer, H .
AMERICAN JOURNAL OF PATHOLOGY, 1999, 155 (03) :907-913
[5]   Podocyte cell cycle regulation and proliferation in collapsing glomerulopathies [J].
Barisoni, L ;
Mokrzycki, M ;
Sablay, L ;
Nagata, M ;
Yamase, H ;
Mundel, P .
KIDNEY INTERNATIONAL, 2000, 58 (01) :137-143
[6]   NPHS2, encoding the glomerular protein podocin, is mutated in autosomal recessive steroid-resistant nephrotic syndrome [J].
Boute, N ;
Gribouval, O ;
Roselli, S ;
Benessy, F ;
Lee, H ;
Fuchshuber, A ;
Dahan, K ;
Gubler, MC ;
Niaudet, P ;
Antignac, C .
NATURE GENETICS, 2000, 24 (04) :349-354
[7]  
Haltia A, 1999, EXP NEPHROL, V7, P52
[8]   Regulation of cell adhesion and anchorage-dependent growth by a new beta(1)-integrin-linked protein kinase [J].
Hannigan, GE ;
LeungHagesteijn, C ;
FitzGibbon, L ;
Coppolino, MG ;
Radeva, G ;
Filmus, J ;
Bell, JC ;
Dedhar, S .
NATURE, 1996, 379 (6560) :91-96
[9]   Real time quantitative PCR [J].
Heid, CA ;
Stevens, J ;
Livak, KJ ;
Williams, PM .
GENOME RESEARCH, 1996, 6 (10) :986-994
[10]   Nephrin localizes at the podocyte filtration slit area and is characteristically spliced in the human kidney [J].
Holthöfer, H ;
Ahola, H ;
Solin, ML ;
Wang, SX ;
Palmen, T ;
Luimula, P ;
Miettinen, A ;
Kerjaschki, D .
AMERICAN JOURNAL OF PATHOLOGY, 1999, 155 (05) :1681-1687