Amplified fragment length polymorphism to detect clonal diversity and distribution of Mycobacterium avium subspecies paratuberculosis in selected Minnesota dairy cattle

被引：5
作者：
Kiehnbaum, LA
Amonsin, A
Wells, SJ
Kapur, V
机构：
[1] Univ Minnesota, Ctr Anim Hlth & Food Safety, St Paul, MN 55108 USA
[2] Univ Minnesota, Biomed Genom Ctr, St Paul, MN 55108 USA
[3] Chulalongkorn Univ, Fac Vet Sci, Dept Vet Sci, Bangkok 10330, Thailand
[4] Ctr Dis Control & Prevent, Minneapolis, MN 55414 USA
来源：
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2005年 / 17卷 / 04期
关键词：
amplified fragment length polymorphisms; Johne's disease;
D O I：
10.1177/104063870501700402
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The molecular ecology of Mycobacterium avium subspecies paratuberculosis (MAP), the causative agent of Johne's disease, is not well understood in the United States. In this study, a DNA fingerprinting method, amplified fragment length polymorphism (AFLP), was used to subtype the pathogen and assess the clonal diversity of MAP in Minnesota dairy herds. Fifty-six fecal culture test-positive isolates from various Minnesota counties and culture dates were analyzed in this study. The AFLP identified 11 profiles with 50% of isolates representing I major profile. The major profile was distributed across the state. The genetic diversity of bovine MAP clones in Minnesota based on AFLP analysis of this data appears to be relatively low.
引用
收藏
页码：311 / 315
页数：5
相关论文
共 14 条
[1]   Multilocus short sequence repeat sequencing approach for differentiating among Mycobacterium avium subsp paratuberculosis strains [J].
Amonsin, A ;
Li, LL ;
Zhang, O ;
Bannantine, JP ;
Motiwala, AS ;
Sreevatsan, S ;
Kapur, V .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (04) :1694-1702
[2]  
BELISLE JT, 2000, MYCOBACTERIA PROTOCO, P31
[3]   Characterization of IS900 loci in Mycobacterium avium subsp paratuberculosis and development of multiplex PCR typing [J].
Bull, TJ ;
Hermon-Taylor, J ;
Pavlik, I ;
El-Zaatari, F ;
Tizard, M .
MICROBIOLOGY-SGM, 2000, 146 :2185-2197
[4]   Review article:: Mycobacterium avium subsp paratuberculosis as one cause of Crohn's disease [J].
Chamberlin, W ;
Graham, DY ;
Hulten, K ;
El-Zimaity, HMT ;
Schwartz, MR ;
Naser, S ;
Shafran, I ;
El-Zaatari, FAK .
ALIMENTARY PHARMACOLOGY & THERAPEUTICS, 2001, 15 (03) :337-346
[5]   NUMERICAL INDEX OF THE DISCRIMINATORY ABILITY OF TYPING SYSTEMS - AN APPLICATION OF SIMPSONS INDEX OF DIVERSITY [J].
HUNTER, PR ;
GASTON, MA .
JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (11) :2465-2466
[6]   Molecular epidemiology of Mycobacterium avium subsp paratuberculosis:: Evidence for limited strain diversity, strain sharing, and identification of unique targets for diagnosis [J].
Motiwala, AS ;
Strother, M ;
Amonsin, A ;
Byrum, B ;
Naser, SA ;
Stabel, JR ;
Shulaw, WP ;
Bannantine, JP ;
Kapur, V ;
Sreevatsan, S .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (05) :2015-2026
[7]  
National Animal Health Monitoring System, 1997, JOHN DIS US DAIR OP
[8]   Characterization of novel coding sequences specific to Mycobacterium avium subsp paratuberculosis:: Implications for diagnosis of Johne's disease [J].
Paustian, ML ;
Amonsin, A ;
Kapur, V ;
Bannantine, JP .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (06) :2675-2681
[9]   Standardisation of restriction fragment length polymorphism analysis for Mycobacterium avium subspecies paratuberculosis [J].
Pavlik, I ;
Horvathova, A ;
Dvorska, L ;
Bartl, J ;
Svastova, P ;
du Maine, R ;
Rychlik, I .
JOURNAL OF MICROBIOLOGICAL METHODS, 1999, 38 (1-2) :155-167
[10]  
Pavlik I, 1999, PROCEEDINGS OF THE SIXTH INTERNATIONAL COLLOQUIUM ON PARATUBERCULOSIS, P321
12