Partition of Glucose Oxidase from Aspergillus niger in Aqueous Two-Phase Systems Based on Salt and Polyethylene Glycol

被引：5
|
作者：
Singh, Jagdish [1 ]
Verma, Neelam [1 ]
机构：
[1] Punjabi Univ Patiala, Dept Biotechnol, Patiala 147002, Punjab, India
来源：
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 2010年 / 53卷 / 05期
关键词：
Aspergillus niger; glucose oxidase; aqueous two phase; PURIFICATION; MECHANISM; PROTEINS;
D O I：
10.1590/S1516-89132010000500007
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The aim of this work was to study the isolation of glucose oxidase (GOx) from Aspergillus niger in aqueous two phase system consisting of PEG 7500 (150g l(-1)), potassium phosphate (175 g l(-1), K(2)HPO(4) + KH(2)PO(4)) and glucose (10 gl(-1)), the enzyme was partitioned in polymer phase. By sequential extraction GOx (69.2%) was recovered in polymer phase by 11.8 fold purification, giving a yield of 129U mg protein-(1).
引用
收藏
页码：1051 / 1056
页数：6
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