Isolation and characterization of the human DC-SIGN and DC-SIGNR promoters

被引:32
作者
Liu, HB [1 ]
Yu, WD [1 ]
Liou, LY [1 ]
Rice, AP [1 ]
机构
[1] Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA
关键词
DC-SIGN; DC-SIGNR; dendritic cells; promoter;
D O I
10.1016/S0378-1119(03)00674-7
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
DC-SIGN is a C-type lectin expressed on the surface of dendritic cells (DCs) that is used by a number of human pathogens to disseminate infection in the host. In the human genome, there is a gene closely related to DC-SIGN, termed DC-SIGNR (also L-SIGN, DC-SIGN2), which likely arose through gene duplication. DC-SIGN protein and RNA expression is largely restricted to DCs and some specialized macrophages in lung and placenta, while DC-SIGNR expression is largely restricted to lymph nodes and liver sinusoidal endothelial cells. To begin to investigate the cell type-restricted expression of these closely related genes, we isolated the human DC-SIGN and DC-SIGNR promoters. They were found to be relatively weak promoters that express similarly in plasmid transfection assays in several transformed cell lines, suggesting that the cis-regulatory elements that confer cell-type restricted expression of these two genes are located outside of the promoters. The DC-SIGN gene contains four major transcriptional start sites at + 1, + 271, + 364, and + 435, with the + 364 site being the most abundantly expressed in DCs. The DC-SIGN promoter is contained within nucleotides + 251 to + 487. AP-1, Sp1, Ets-1, and NF-KB binding sites in the DC-SIGN promoter appear to be important for function. Thus, despite its highly restricted pattern of expression, the DC-SIGN promoter has features common to promoters that are active in other cell types. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:149 / 159
页数:11
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